Results 21 - 34 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1349 | 5' | -59.2 | NC_001335.1 | + | 25201 | 0.68 | 0.341433 |
Target: 5'- aAGaaGGCaGC-CGc-CAGCGCCAUCACg -3' miRNA: 3'- -UCggCCG-CGaGCuaGUCGCGGUAGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 20743 | 0.66 | 0.484354 |
Target: 5'- uGGCCgacggggcgaugaaGGCGUUCacgcuGUCAGCguucgcaGCCAUCGCa -3' miRNA: 3'- -UCGG--------------CCGCGAGc----UAGUCG-------CGGUAGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 35416 | 0.66 | 0.458465 |
Target: 5'- gAGaUCGGCgGCUCGuagaGGUaGCCAUCGCg -3' miRNA: 3'- -UC-GGCCG-CGAGCuag-UCG-CGGUAGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 37854 | 0.66 | 0.458465 |
Target: 5'- -uCgGGCGUUCGG-CAGCGUCuUCGCc -3' miRNA: 3'- ucGgCCGCGAGCUaGUCGCGGuAGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 49218 | 0.66 | 0.439062 |
Target: 5'- cGUCGuacGCGCUCGGguugCcGCGCCAgucgUCGCg -3' miRNA: 3'- uCGGC---CGCGAGCUa---GuCGCGGU----AGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 24135 | 0.67 | 0.420137 |
Target: 5'- uGCUGGCGCUCGcggccaGGCGUgGuucuUCGCg -3' miRNA: 3'- uCGGCCGCGAGCuag---UCGCGgU----AGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 27288 | 0.67 | 0.410861 |
Target: 5'- cGCCucGGCGaggUUGGccUCGGCGCgGUCACg -3' miRNA: 3'- uCGG--CCGCg--AGCU--AGUCGCGgUAGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 16211 | 0.67 | 0.401714 |
Target: 5'- uGGCCGGUcuGCUggcCGGUC--UGCCGUCGCu -3' miRNA: 3'- -UCGGCCG--CGA---GCUAGucGCGGUAGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 16256 | 0.68 | 0.375062 |
Target: 5'- gAGCCGGCGCU-----GGCGUaGUCGCg -3' miRNA: 3'- -UCGGCCGCGAgcuagUCGCGgUAGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 8746 | 0.69 | 0.310038 |
Target: 5'- cGGCCGGuCGUUcaucaCGAUCAGCaaGCCAgaccCGCa -3' miRNA: 3'- -UCGGCC-GCGA-----GCUAGUCG--CGGUa---GUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 13702 | 0.7 | 0.273952 |
Target: 5'- cGuuGGCGCUUGAguUCGgccacGCGCCGUCu- -3' miRNA: 3'- uCggCCGCGAGCU--AGU-----CGCGGUAGug -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 52069 | 0.7 | 0.259829 |
Target: 5'- aGGCCGGCuCUCGcaucgucgcauGUCAGCGCggguaugggcgugUAUCGCa -3' miRNA: 3'- -UCGGCCGcGAGC-----------UAGUCGCG-------------GUAGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 9307 | 0.72 | 0.208669 |
Target: 5'- gAGCUGGCcaacuucaccaacgcGCUCGAUCAGauCGCCAaacaggUCGCu -3' miRNA: 3'- -UCGGCCG---------------CGAGCUAGUC--GCGGU------AGUG- -5' |
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1349 | 5' | -59.2 | NC_001335.1 | + | 3706 | 0.75 | 0.113719 |
Target: 5'- --gCGGCGCUCGuucgaugagGUCGGUGUCGUCACg -3' miRNA: 3'- ucgGCCGCGAGC---------UAGUCGCGGUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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