Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1362 | 5' | -59.6 | NC_001335.1 | + | 18615 | 0.68 | 0.350132 |
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Target: 5'- gCCAGgAGCGCGGCCcCuGCUUCGcgucguUGAg -3' miRNA: 3'- -GGUCgUCGCGCUGGuGcCGGAGC------ACU- -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 27291 | 0.69 | 0.34207 |
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Target: 5'- cUCGGCgagguuggccucGGCGCGGUCACgGGCCUCGUc- -3' miRNA: 3'- -GGUCG------------UCGCGCUGGUG-CCGGAGCAcu -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 41418 | 0.69 | 0.318694 |
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Target: 5'- gCCGGggcCGGCGCaGACCAggacuagcUGGCCUCGUcGGa -3' miRNA: 3'- -GGUC---GUCGCG-CUGGU--------GCCGGAGCA-CU- -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 16623 | 0.71 | 0.255858 |
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Target: 5'- gCCGGUAGCGagcacagGGCCGaGGCC-CGUGAa -3' miRNA: 3'- -GGUCGUCGCg------CUGGUgCCGGaGCACU- -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 24128 | 0.71 | 0.24954 |
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Target: 5'- gCGGCGGUGCuGGCgcuCGCGGCCaggCGUGGu -3' miRNA: 3'- gGUCGUCGCG-CUG---GUGCCGGa--GCACU- -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 45031 | 0.71 | 0.243352 |
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Target: 5'- cCCAGUGGgaGaCGGCCACGGCgUCGggGAa -3' miRNA: 3'- -GGUCGUCg-C-GCUGGUGCCGgAGCa-CU- -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 16424 | 0.76 | 0.113237 |
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Target: 5'- aCCuGCAGaaCGUGGCCucgGGCCUCGUGAa -3' miRNA: 3'- -GGuCGUC--GCGCUGGug-CCGGAGCACU- -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 19365 | 1.1 | 0.000313 |
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Target: 5'- cCCAGCAGCGCGACCACGGCCUCGUGAg -3' miRNA: 3'- -GGUCGUCGCGCUGGUGCCGGAGCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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