Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1362 | 5' | -59.6 | NC_001335.1 | + | 49167 | 0.67 | 0.447385 |
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Target: 5'- cUCAGUAGuCGCuGugCugGGUCUCGa-- -3' miRNA: 3'- -GGUCGUC-GCG-CugGugCCGGAGCacu -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 34335 | 0.66 | 0.456953 |
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Target: 5'- aCGGCAGCG-GGCCGCuGGCCa----- -3' miRNA: 3'- gGUCGUCGCgCUGGUG-CCGGagcacu -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 46415 | 0.66 | 0.476413 |
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Target: 5'- cCCGcCGGC-CGAuuCCACGGCCUUGUu- -3' miRNA: 3'- -GGUcGUCGcGCU--GGUGCCGGAGCAcu -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 32919 | 0.66 | 0.476413 |
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Target: 5'- -gGGCGGUGCu-CCACGGUCgUCGUa- -3' miRNA: 3'- ggUCGUCGCGcuGGUGCCGG-AGCAcu -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 32513 | 0.66 | 0.476413 |
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Target: 5'- aUCAGCAGUGUGACgCGCaauGGCC-CGa-- -3' miRNA: 3'- -GGUCGUCGCGCUG-GUG---CCGGaGCacu -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 48476 | 0.66 | 0.486296 |
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Target: 5'- uCCAGU-GUGCGA--ACGGCCUUGUa- -3' miRNA: 3'- -GGUCGuCGCGCUggUGCCGGAGCAcu -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 33131 | 0.66 | 0.496275 |
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Target: 5'- aUCGGCuGCGacauCCuCGGCCUCGUa- -3' miRNA: 3'- -GGUCGuCGCgcu-GGuGCCGGAGCAcu -5' |
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| 1362 | 5' | -59.6 | NC_001335.1 | + | 52127 | 0.66 | 0.506346 |
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Target: 5'- gCUAGCcGCGUG-CCGCGGCgCUCu--- -3' miRNA: 3'- -GGUCGuCGCGCuGGUGCCG-GAGcacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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