Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13941 | 3' | -52.7 | NC_003521.1 | + | 204200 | 0.66 | 0.991184 |
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Target: 5'- cGAgggCGUcAGGCagCCGUGGUGGAagagGCUu -3' miRNA: 3'- aCUa--GCA-UUCGg-GGCAUCACCUa---CGG- -5' |
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| 13941 | 3' | -52.7 | NC_003521.1 | + | 213706 | 0.8 | 0.478286 |
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Target: 5'- aGAUUGUGGGCCUCGg---GGAUGCCc -3' miRNA: 3'- aCUAGCAUUCGGGGCaucaCCUACGG- -5' |
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| 13941 | 3' | -52.7 | NC_003521.1 | + | 213734 | 0.66 | 0.994136 |
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Target: 5'- aGA-CGgc-GCCgcagaaggCCGUGGUGGcgGCCa -3' miRNA: 3'- aCUaGCauuCGG--------GGCAUCACCuaCGG- -5' |
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| 13941 | 3' | -52.7 | NC_003521.1 | + | 216491 | 0.67 | 0.989974 |
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Target: 5'- cGcUCGUAGGCCUCGUcugcGUG--UGCCa -3' miRNA: 3'- aCuAGCAUUCGGGGCAu---CACcuACGG- -5' |
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| 13941 | 3' | -52.7 | NC_003521.1 | + | 221111 | 0.7 | 0.934561 |
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Target: 5'- gUGAUCGUGAcCUCCGUGGUG--UGCg -3' miRNA: 3'- -ACUAGCAUUcGGGGCAUCACcuACGg -5' |
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| 13941 | 3' | -52.7 | NC_003521.1 | + | 222189 | 0.69 | 0.966597 |
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Target: 5'- cGAggcgccgCGgcAGCCCCGaagGGUGGc-GCCg -3' miRNA: 3'- aCUa------GCauUCGGGGCa--UCACCuaCGG- -5' |
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| 13941 | 3' | -52.7 | NC_003521.1 | + | 235231 | 0.66 | 0.991184 |
|
Target: 5'- ---aCGUAGGCCUCGUugguccgcaGGcUGGA-GCCg -3' miRNA: 3'- acuaGCAUUCGGGGCA---------UC-ACCUaCGG- -5' |
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| 13941 | 3' | -52.7 | NC_003521.1 | + | 236693 | 0.74 | 0.809943 |
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Target: 5'- cGGUUGUGgaacGGCCUCGUcuGGUugGGGUGCCu -3' miRNA: 3'- aCUAGCAU----UCGGGGCA--UCA--CCUACGG- -5' |
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| 13941 | 3' | -52.7 | NC_003521.1 | + | 236858 | 0.71 | 0.92426 |
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Target: 5'- -uGUCG-GAGCCCCGaGGcUGGcUGCCg -3' miRNA: 3'- acUAGCaUUCGGGGCaUC-ACCuACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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