Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13954 | 3' | -53.3 | NC_003521.1 | + | 24593 | 1.1 | 0.008639 |
|
Target: 5'- gCCGCCGAUUCAUCGGAUCGUCGCUGUg -3' miRNA: 3'- -GGCGGCUAAGUAGCCUAGCAGCGACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 20625 | 0.7 | 0.943108 |
|
Target: 5'- aCGCCGAggaccucgUCGUCGGAcaCGUUGgaGUa -3' miRNA: 3'- gGCGGCUa-------AGUAGCCUa-GCAGCgaCA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 16945 | 0.66 | 0.99175 |
|
Target: 5'- aCCGCCGuuacCGUCGGGga-UUGCUGg -3' miRNA: 3'- -GGCGGCuaa-GUAGCCUagcAGCGACa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 15459 | 0.7 | 0.938575 |
|
Target: 5'- uCCGCCGAUUCGUCuaacuuucccuuGGAauuUCGUgGCg-- -3' miRNA: 3'- -GGCGGCUAAGUAG------------CCU---AGCAgCGaca -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 5554 | 0.67 | 0.986494 |
|
Target: 5'- gCCGCCGccaUCGUUGuuccGUcCGUCGCUGUc -3' miRNA: 3'- -GGCGGCua-AGUAGCc---UA-GCAGCGACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 1790 | 0.69 | 0.951105 |
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Target: 5'- aCCGCCauccccuGAggCGuUCGuGGUCGUUGCUGUg -3' miRNA: 3'- -GGCGG-------CUaaGU-AGC-CUAGCAGCGACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 1327 | 0.71 | 0.923622 |
|
Target: 5'- cCCGCCGAUg-GUCGG-UCGa-GCUGUa -3' miRNA: 3'- -GGCGGCUAagUAGCCuAGCagCGACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 450 | 0.68 | 0.976694 |
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Target: 5'- gCCgGCCGGUcggacguguuUCGggccggCGGGUCGUCGCgGg -3' miRNA: 3'- -GG-CGGCUA----------AGUa-----GCCUAGCAGCGaCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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