Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13963 | 3' | -61.3 | NC_003521.1 | + | 2322 | 0.68 | 0.662428 |
Target: 5'- gCUGCgCgccgGCCuCCuGGGGCUGCugACCCUg -3' miRNA: 3'- -GACGgGa---UGG-GGcUCCCGAUG--UGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 5910 | 0.66 | 0.765162 |
Target: 5'- aCUGCUgUACCgCGAGGGgCUACGaugacgguUCCg -3' miRNA: 3'- -GACGGgAUGGgGCUCCC-GAUGU--------GGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 6051 | 0.71 | 0.510322 |
Target: 5'- gUGCCCcagcgGCCCCGcAGGGCgugaucACGCCa- -3' miRNA: 3'- gACGGGa----UGGGGC-UCCCGa-----UGUGGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 13958 | 0.7 | 0.527852 |
Target: 5'- gCUGCCCUcgcuggaGCCCUGAuGGGCcu-GCCCg -3' miRNA: 3'- -GACGGGA-------UGGGGCU-CCCGaugUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 14220 | 0.68 | 0.65281 |
Target: 5'- -aGCCCcugACgCCGcuGGGCUACGCCg- -3' miRNA: 3'- gaCGGGa--UGgGGCu-CCCGAUGUGGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 16190 | 0.66 | 0.791408 |
Target: 5'- cCUGUCCgagugcugGCCCuuCGAGguGGCgGCGCCCUg -3' miRNA: 3'- -GACGGGa-------UGGG--GCUC--CCGaUGUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 16803 | 0.66 | 0.774026 |
Target: 5'- -cGCCC-GCCCCGAccgccGGGCccgccGCugCCg -3' miRNA: 3'- gaCGGGaUGGGGCU-----CCCGa----UGugGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 18955 | 0.66 | 0.803276 |
Target: 5'- -cGCCCUACCCagcgacaacgaggaGGGGGUcgaggACGCgCCg -3' miRNA: 3'- gaCGGGAUGGGg-------------CUCCCGa----UGUG-GGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 21242 | 0.7 | 0.54748 |
Target: 5'- cCUGCCCUuggaauggACCCuCGAGGaGCUG-GCCCc -3' miRNA: 3'- -GACGGGA--------UGGG-GCUCC-CGAUgUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 25401 | 0.69 | 0.585451 |
Target: 5'- -gGCUCUGCUUCacgGAGGGCU-CGCCCa -3' miRNA: 3'- gaCGGGAUGGGG---CUCCCGAuGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 26581 | 0.71 | 0.483157 |
Target: 5'- gCUGCUgUGCCUcuacacggucuuCGuGGGCUGCAUCCUg -3' miRNA: 3'- -GACGGgAUGGG------------GCuCCCGAUGUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 29933 | 0.67 | 0.737972 |
Target: 5'- -gGCCUggcacgGCCaCCGAGGcGacgUACACCCUg -3' miRNA: 3'- gaCGGGa-----UGG-GGCUCC-Cg--AUGUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 35969 | 0.66 | 0.756193 |
Target: 5'- gUGCCCgagaUCCGGGuGGaCUACACCUc -3' miRNA: 3'- gACGGGaug-GGGCUC-CC-GAUGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 39599 | 0.67 | 0.710046 |
Target: 5'- -gGUCCUGCCCUGGGuGGCgGCGuCUCg -3' miRNA: 3'- gaCGGGAUGGGGCUC-CCGaUGU-GGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 42788 | 0.69 | 0.585451 |
Target: 5'- cCUGCCCgUGCagCCCGGcGGCUGCACgCg -3' miRNA: 3'- -GACGGG-AUG--GGGCUcCCGAUGUGgGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 54322 | 0.74 | 0.344739 |
Target: 5'- -cGCCCgagAUCCCGAcgGGGCUcuCGCCCUg -3' miRNA: 3'- gaCGGGa--UGGGGCU--CCCGAu-GUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 55630 | 0.66 | 0.765162 |
Target: 5'- -gGCCC-ACaCCGAGGGCgucACGCuCCa -3' miRNA: 3'- gaCGGGaUGgGGCUCCCGa--UGUG-GGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 59229 | 0.67 | 0.719423 |
Target: 5'- -gGCCCUggacGCCCUGgaggugaacguGGGGCUGCugCg- -3' miRNA: 3'- gaCGGGA----UGGGGC-----------UCCCGAUGugGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 59514 | 0.68 | 0.691122 |
Target: 5'- -cGCCCUGgCCCGcGGGCg--GCCUg -3' miRNA: 3'- gaCGGGAUgGGGCuCCCGaugUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 60734 | 0.66 | 0.765162 |
Target: 5'- gCUGCCCU-CCCUGgacGGGGUgACGCgCa -3' miRNA: 3'- -GACGGGAuGGGGC---UCCCGaUGUGgGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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