Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13963 | 3' | -61.3 | NC_003521.1 | + | 234284 | 0.74 | 0.350582 |
Target: 5'- -cGCCCUGCCCUG-GGGCgggacgcagacCGCCCUc -3' miRNA: 3'- gaCGGGAUGGGGCuCCCGau---------GUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 232554 | 0.66 | 0.765162 |
Target: 5'- gUGgCCUACUaCGGGGGCUGCugUg- -3' miRNA: 3'- gACgGGAUGGgGCUCCCGAUGugGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 55630 | 0.66 | 0.765162 |
Target: 5'- -gGCCC-ACaCCGAGGGCgucACGCuCCa -3' miRNA: 3'- gaCGGGaUGgGGCUCCCGa--UGUG-GGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 60734 | 0.66 | 0.765162 |
Target: 5'- gCUGCCCU-CCCUGgacGGGGUgACGCgCa -3' miRNA: 3'- -GACGGGAuGGGGC---UCCCGaUGUGgGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 138846 | 0.67 | 0.747127 |
Target: 5'- -gGCCUgcgGCCa-GAuGGGCUGCACCUUc -3' miRNA: 3'- gaCGGGa--UGGggCU-CCCGAUGUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 59229 | 0.67 | 0.719423 |
Target: 5'- -gGCCCUggacGCCCUGgaggugaacguGGGGCUGCugCg- -3' miRNA: 3'- gaCGGGA----UGGGGC-----------UCCCGAUGugGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 79595 | 0.67 | 0.716616 |
Target: 5'- aCUGCCgCUGCCgCCGccaucgaacuugccGGGGCgaccgcgGCGCCUc -3' miRNA: 3'- -GACGG-GAUGG-GGC--------------UCCCGa------UGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 39599 | 0.67 | 0.710046 |
Target: 5'- -gGUCCUGCCCUGGGuGGCgGCGuCUCg -3' miRNA: 3'- gaCGGGAUGGGGCUC-CCGaUGU-GGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 152357 | 0.68 | 0.681591 |
Target: 5'- gCUGCUCUgggGCCggCCG-GGGCggcGCGCCCUc -3' miRNA: 3'- -GACGGGA---UGG--GGCuCCCGa--UGUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 67685 | 0.68 | 0.672023 |
Target: 5'- -aGCCCaagUACgCCGAGGuCUACGCCUc -3' miRNA: 3'- gaCGGG---AUGgGGCUCCcGAUGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 202550 | 0.68 | 0.662428 |
Target: 5'- gCUGCgCgccgGCCuCCuGGGGCUGCugACCCUg -3' miRNA: 3'- -GACGgGa---UGG-GGcUCCCGAUG--UGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 79215 | 0.68 | 0.65281 |
Target: 5'- -aGCaCgUugCCCGAGGGCaccagAUGCCCg -3' miRNA: 3'- gaCG-GgAugGGGCUCCCGa----UGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 136134 | 0.69 | 0.633538 |
Target: 5'- -cGCUCUACCCCGuGGcGCgcCGCCa- -3' miRNA: 3'- gaCGGGAUGGGGCuCC-CGauGUGGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 236596 | 0.69 | 0.633538 |
Target: 5'- -aGCCa-GCCUCG-GGGCUccgACACCCUu -3' miRNA: 3'- gaCGGgaUGGGGCuCCCGA---UGUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 91758 | 0.69 | 0.604638 |
Target: 5'- cCUaCCCUACCCCGcGGGUcccGCAUCCc -3' miRNA: 3'- -GAcGGGAUGGGGCuCCCGa--UGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 42788 | 0.69 | 0.585451 |
Target: 5'- cCUGCCCgUGCagCCCGGcGGCUGCACgCg -3' miRNA: 3'- -GACGGG-AUG--GGGCUcCCGAUGUGgGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 111746 | 0.7 | 0.538103 |
Target: 5'- cCUGCagUACgCCGAGGGuCUGCGCCa- -3' miRNA: 3'- -GACGggAUGgGGCUCCC-GAUGUGGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 222095 | 0.7 | 0.516753 |
Target: 5'- -cGCCCUGCagcgccggcagcguCUCGAGGcGCUGCGCCg- -3' miRNA: 3'- gaCGGGAUG--------------GGGCUCC-CGAUGUGGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 112448 | 0.71 | 0.492137 |
Target: 5'- -gGCCCaggcgGCCCUGGuGGGC-GCGCCCg -3' miRNA: 3'- gaCGGGa----UGGGGCU-CCCGaUGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 130149 | 0.74 | 0.337535 |
Target: 5'- gCUGCC--GCCaCCGAGcGCUACGCCCUg -3' miRNA: 3'- -GACGGgaUGG-GGCUCcCGAUGUGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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