Results 1 - 20 of 71 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13966 | 5' | -53.4 | NC_003521.1 | + | 196604 | 0.66 | 0.990457 |
Target: 5'- cUGCACGCGcuggaaaUCGAGcUGCaGCCGcCGUc -3' miRNA: 3'- -ACGUGUGCa------AGUUCaACG-CGGC-GCAc -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 65289 | 0.66 | 0.990457 |
Target: 5'- cUGCACGCGggcCAGGaUGCGCgugcuaggggCGCGg- -3' miRNA: 3'- -ACGUGUGCaa-GUUCaACGCG----------GCGCac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 143288 | 0.66 | 0.990457 |
Target: 5'- cGCGCAUGUUCGA---GCGCCuCGa- -3' miRNA: 3'- aCGUGUGCAAGUUcaaCGCGGcGCac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 29597 | 0.66 | 0.990084 |
Target: 5'- aGCGCACGUagucggugCAaaugaaagcguccuGGUUGCGCacguaGCGUa -3' miRNA: 3'- aCGUGUGCAa-------GU--------------UCAACGCGg----CGCAc -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 26270 | 0.66 | 0.989167 |
Target: 5'- cUGCACAUGggUCugcg-GCGCUGCGg- -3' miRNA: 3'- -ACGUGUGCa-AGuucaaCGCGGCGCac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 127663 | 0.66 | 0.989167 |
Target: 5'- uUGCGCAgGaUCAGGaagGCGuCCGUGUu -3' miRNA: 3'- -ACGUGUgCaAGUUCaa-CGC-GGCGCAc -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 204649 | 0.66 | 0.989167 |
Target: 5'- gGC-CGCGgagaCGAGUUGCGCuCGCu-- -3' miRNA: 3'- aCGuGUGCaa--GUUCAACGCG-GCGcac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 60071 | 0.66 | 0.987745 |
Target: 5'- aGCACGCGgUCca---GCGCCGUGUc -3' miRNA: 3'- aCGUGUGCaAGuucaaCGCGGCGCAc -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 66937 | 0.66 | 0.987745 |
Target: 5'- gGCaACGuggUGUUCAAGUaccugGCGCUGCGg- -3' miRNA: 3'- aCG-UGU---GCAAGUUCAa----CGCGGCGCac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 131851 | 0.66 | 0.987292 |
Target: 5'- gUGgGCugGUUCAAGgcggccacggccaucGUGCCGCGc- -3' miRNA: 3'- -ACgUGugCAAGUUCaa-------------CGCGGCGCac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 26056 | 0.66 | 0.986183 |
Target: 5'- aGCugAUGgu-GGGcUGCaGCCGCGUGg -3' miRNA: 3'- aCGugUGCaagUUCaACG-CGGCGCAC- -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 85936 | 0.66 | 0.986183 |
Target: 5'- --aGCACGUUCuc---GCGCCGCGa- -3' miRNA: 3'- acgUGUGCAAGuucaaCGCGGCGCac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 153394 | 0.66 | 0.986183 |
Target: 5'- aGCGCAggaucuccuCGUgCAGG-UGCGCCaccgGCGUGg -3' miRNA: 3'- aCGUGU---------GCAaGUUCaACGCGG----CGCAC- -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 101804 | 0.66 | 0.986183 |
Target: 5'- cGCGCACGaagccCAGGggGCGgUCGCGg- -3' miRNA: 3'- aCGUGUGCaa---GUUCaaCGC-GGCGCac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 114836 | 0.67 | 0.980575 |
Target: 5'- cGCGCAcCGUgcUCAuGcUGCGCCuuaGCGUGc -3' miRNA: 3'- aCGUGU-GCA--AGUuCaACGCGG---CGCAC- -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 123574 | 0.67 | 0.980575 |
Target: 5'- gGCAguCGUgCA----GCGCCGCGUGa -3' miRNA: 3'- aCGUguGCAaGUucaaCGCGGCGCAC- -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 223656 | 0.67 | 0.980575 |
Target: 5'- cGcCACACGgcccgcgUCGGG--GCGCCGCGa- -3' miRNA: 3'- aC-GUGUGCa------AGUUCaaCGCGGCGCac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 63920 | 0.67 | 0.980575 |
Target: 5'- aGCuCugGUUCAcgggcacggGGUUGCGCgUGCGg- -3' miRNA: 3'- aCGuGugCAAGU---------UCAACGCG-GCGCac -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 229810 | 0.67 | 0.980575 |
Target: 5'- gGCugACGUUCA--UUGCGgCCG-GUGc -3' miRNA: 3'- aCGugUGCAAGUucAACGC-GGCgCAC- -5' |
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13966 | 5' | -53.4 | NC_003521.1 | + | 168584 | 0.67 | 0.980575 |
Target: 5'- aGCACAUcUUCGAGgagguggcGCGCCucauGCGUGu -3' miRNA: 3'- aCGUGUGcAAGUUCaa------CGCGG----CGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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