Results 1 - 20 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13972 | 5' | -55.4 | NC_003521.1 | + | 78493 | 0.74 | 0.647519 |
Target: 5'- -cCCGCagUCGCCGCCUGCGGGUAgUa- -3' miRNA: 3'- uaGGCG--AGUGGUGGAUGCUCAUgGac -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 145091 | 0.66 | 0.951608 |
Target: 5'- -gUgGCUCAgCCGCCUGCuGG-ACCUGg -3' miRNA: 3'- uaGgCGAGU-GGUGGAUGcUCaUGGAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 134943 | 0.66 | 0.951608 |
Target: 5'- -gCCGuCUUGCgCACCUGCGAGUugaGgCUGa -3' miRNA: 3'- uaGGC-GAGUG-GUGGAUGCUCA---UgGAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 119875 | 0.67 | 0.949164 |
Target: 5'- gGUCCGCagucgguucuccucgUCGCC-CC-GCGGGUggGCCUGg -3' miRNA: 3'- -UAGGCG---------------AGUGGuGGaUGCUCA--UGGAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 111498 | 0.67 | 0.94749 |
Target: 5'- --gCGCUCaACCGCCUGCuGGGcgACCUc -3' miRNA: 3'- uagGCGAG-UGGUGGAUG-CUCa-UGGAc -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 44067 | 0.67 | 0.94749 |
Target: 5'- gAUCCGCaacCcCUACCUGCGGGUcaaGCCg- -3' miRNA: 3'- -UAGGCGa--GuGGUGGAUGCUCA---UGGac -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 136152 | 0.67 | 0.938577 |
Target: 5'- -gCCGC-CACCACCUGCuGGaGCCc- -3' miRNA: 3'- uaGGCGaGUGGUGGAUGcUCaUGGac -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 143454 | 0.67 | 0.933777 |
Target: 5'- -gCCGCUCgACCGCCaGCGcGUGCUc- -3' miRNA: 3'- uaGGCGAG-UGGUGGaUGCuCAUGGac -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 112899 | 0.68 | 0.912273 |
Target: 5'- -gCCGCgagaUCGCCGCCUGgGAGgGCCc- -3' miRNA: 3'- uaGGCG----AGUGGUGGAUgCUCaUGGac -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 144469 | 0.68 | 0.900145 |
Target: 5'- uUCgGCgCGCCaccaccauaACCUGCGAGgaggACCUGg -3' miRNA: 3'- uAGgCGaGUGG---------UGGAUGCUCa---UGGAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 120042 | 0.68 | 0.893744 |
Target: 5'- gGUCgCGCUCGcCCACC-GCGcAGUGCCc- -3' miRNA: 3'- -UAG-GCGAGU-GGUGGaUGC-UCAUGGac -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 144121 | 0.69 | 0.887122 |
Target: 5'- -aCCGC-CGCgGCCUggACGAGgugcGCCUGg -3' miRNA: 3'- uaGGCGaGUGgUGGA--UGCUCa---UGGAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 137631 | 0.69 | 0.880282 |
Target: 5'- -aCCGCUCGCgCAUCgcgguCGAGUACgUGc -3' miRNA: 3'- uaGGCGAGUG-GUGGau---GCUCAUGgAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 121618 | 0.69 | 0.87323 |
Target: 5'- --gCGCUCACCACCUgGCGcAGcGCCa- -3' miRNA: 3'- uagGCGAGUGGUGGA-UGC-UCaUGGac -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 29197 | 0.7 | 0.850842 |
Target: 5'- -gCC-CUCGCUGCgCaACGAGUACCUGg -3' miRNA: 3'- uaGGcGAGUGGUG-GaUGCUCAUGGAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 112786 | 0.7 | 0.83495 |
Target: 5'- -cCUGCUgGCCGCCUACGGGcacgcGCuCUGg -3' miRNA: 3'- uaGGCGAgUGGUGGAUGCUCa----UG-GAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 60609 | 0.71 | 0.792236 |
Target: 5'- --aCGCgcggCACCACCaacgccgagcGCGAGUACCUGu -3' miRNA: 3'- uagGCGa---GUGGUGGa---------UGCUCAUGGAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 43255 | 0.71 | 0.792236 |
Target: 5'- cAUCCGCcucaUCGCCAuCCUGCGucacUACCUGc -3' miRNA: 3'- -UAGGCG----AGUGGU-GGAUGCuc--AUGGAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 223822 | 0.72 | 0.736483 |
Target: 5'- -gCCGC-CGCCACC-GCGGGcGCCUGc -3' miRNA: 3'- uaGGCGaGUGGUGGaUGCUCaUGGAC- -5' |
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13972 | 5' | -55.4 | NC_003521.1 | + | 36350 | 0.75 | 0.577321 |
Target: 5'- cGUCCuaUCACCugacgGCCUACGAGcacUACCUGa -3' miRNA: 3'- -UAGGcgAGUGG-----UGGAUGCUC---AUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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