Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13976 | 5' | -61.1 | NC_003521.1 | + | 91699 | 0.67 | 0.705827 |
Target: 5'- gGGCgUGCUGCG-GUcugcGUGCC-GCUCc -3' miRNA: 3'- gCCGgACGACGCgCA----CACGGuCGAGu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 29422 | 0.69 | 0.608111 |
Target: 5'- gGGCCccggugacaGCUacaaccaGCGCGUGgGCCAGUUCAu -3' miRNA: 3'- gCCGGa--------CGA-------CGCGCACaCGGUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 148937 | 0.69 | 0.609082 |
Target: 5'- aGcGCCgUGCUGgCGCGUGUGCU-GCUgGa -3' miRNA: 3'- gC-CGG-ACGAC-GCGCACACGGuCGAgU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 232918 | 0.69 | 0.618798 |
Target: 5'- uGGaCCUGCUG-GUGUGUGUCuuucuacuAGCUCu -3' miRNA: 3'- gCC-GGACGACgCGCACACGG--------UCGAGu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 193265 | 0.68 | 0.647977 |
Target: 5'- aCGGUCgGCUGCggaGCGgGUGCCGGgaCGg -3' miRNA: 3'- -GCCGGaCGACG---CGCaCACGGUCgaGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 73167 | 0.68 | 0.65769 |
Target: 5'- uCGGCCUGCgaccugGaCGCGgagcugGCCGGCaUCGa -3' miRNA: 3'- -GCCGGACGa-----C-GCGCaca---CGGUCG-AGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 53194 | 0.68 | 0.667384 |
Target: 5'- uGGCCaGCgGCGCGcG-GcCCGGCUCGc -3' miRNA: 3'- gCCGGaCGaCGCGCaCaC-GGUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 136773 | 0.68 | 0.677052 |
Target: 5'- uGGCCUacCUGCGCGacaGCgGGCUCAc -3' miRNA: 3'- gCCGGAc-GACGCGCacaCGgUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 73968 | 0.67 | 0.696282 |
Target: 5'- uGuGCCUGCUGUGCGagcucaUGU-CCuGCUCGu -3' miRNA: 3'- gC-CGGACGACGCGC------ACAcGGuCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 80141 | 0.69 | 0.589701 |
Target: 5'- gCGGCCggUGCUGCGCGUccaGCC-GCUgGa -3' miRNA: 3'- -GCCGG--ACGACGCGCAca-CGGuCGAgU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 168609 | 0.69 | 0.589701 |
Target: 5'- uGGgCUGCUGgGCGUGcGCCAucuGCUg- -3' miRNA: 3'- gCCgGACGACgCGCACaCGGU---CGAgu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 367 | 0.69 | 0.579086 |
Target: 5'- gCGGCgUGCUGUggaacgaaggugaGUGUGUGCUGGCgcgCGg -3' miRNA: 3'- -GCCGgACGACG-------------CGCACACGGUCGa--GU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 109572 | 0.77 | 0.226669 |
Target: 5'- uCGGCUaGCaUGCGCGUGaagugcGCCAGCUCGg -3' miRNA: 3'- -GCCGGaCG-ACGCGCACa-----CGGUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 122221 | 0.73 | 0.368668 |
Target: 5'- -uGCCUGCgaGCGCGgGUGCCGGCg-- -3' miRNA: 3'- gcCGGACGa-CGCGCaCACGGUCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 105629 | 0.73 | 0.384244 |
Target: 5'- gGGCgUGCUcGgGCGUGUGCgugaagccCAGCUCGg -3' miRNA: 3'- gCCGgACGA-CgCGCACACG--------GUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 126875 | 0.71 | 0.458593 |
Target: 5'- aGGCCUGCgucagugagacgUGCGCGUccGUGgcgauguccuccaCCAGCUCGc -3' miRNA: 3'- gCCGGACG------------ACGCGCA--CAC-------------GGUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 102773 | 0.71 | 0.480829 |
Target: 5'- gCGGCagcgGCUGCGCGaUGacgcgccggcggccGCCAGCUCGg -3' miRNA: 3'- -GCCGga--CGACGCGC-ACa-------------CGGUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 215963 | 0.71 | 0.504496 |
Target: 5'- aGGaCCUGCUGCGagagGUG-GCCGGCg-- -3' miRNA: 3'- gCC-GGACGACGCg---CACaCGGUCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 76969 | 0.7 | 0.532407 |
Target: 5'- gCGGCCga-UGCGCGUGaUGCUGGCg-- -3' miRNA: 3'- -GCCGGacgACGCGCAC-ACGGUCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 145427 | 0.7 | 0.55132 |
Target: 5'- gGGCUUGCUGgGCGccucgguggaccUGUGCUucggcguGCUCAa -3' miRNA: 3'- gCCGGACGACgCGC------------ACACGGu------CGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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