Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13976 | 5' | -61.1 | NC_003521.1 | + | 367 | 0.69 | 0.579086 |
Target: 5'- gCGGCgUGCUGUggaacgaaggugaGUGUGUGCUGGCgcgCGg -3' miRNA: 3'- -GCCGgACGACG-------------CGCACACGGUCGa--GU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 1441 | 0.66 | 0.788259 |
Target: 5'- gGGCCgcgaGCUGCGCcgccgGUGgCAGCa-- -3' miRNA: 3'- gCCGGa---CGACGCGca---CACgGUCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 13981 | 0.68 | 0.667384 |
Target: 5'- gGGCCUGCccgGCGCGgcgGCgGGCg-- -3' miRNA: 3'- gCCGGACGa--CGCGCacaCGgUCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 17968 | 0.66 | 0.788259 |
Target: 5'- aCGGCCUGCUGUGgugcgaguaCGUGUaucGCCAcccGCa-- -3' miRNA: 3'- -GCCGGACGACGC---------GCACA---CGGU---CGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 18430 | 0.73 | 0.361045 |
Target: 5'- gCGGCCUGCUcaagacGCGCGgccUGUGCC-GCUUc -3' miRNA: 3'- -GCCGGACGA------CGCGC---ACACGGuCGAGu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 19665 | 0.66 | 0.761644 |
Target: 5'- gGGgCUGCUGCGC-UGgGCCgAGCcCAa -3' miRNA: 3'- gCCgGACGACGCGcACaCGG-UCGaGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 21294 | 0.69 | 0.579086 |
Target: 5'- gCGGUCUgGCUGCGCGUGgcgGucaucgucaacuuCCAGCUg- -3' miRNA: 3'- -GCCGGA-CGACGCGCACa--C-------------GGUCGAgu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 22356 | 0.68 | 0.65769 |
Target: 5'- gGGCCacUGCgcccacgGCGUG-GUGCCGGC-CAu -3' miRNA: 3'- gCCGG--ACGa------CGCGCaCACGGUCGaGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 22415 | 0.67 | 0.715317 |
Target: 5'- uGGCCaUGCUGCGCGgcgugGCCgAGUa-- -3' miRNA: 3'- gCCGG-ACGACGCGCaca--CGG-UCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 24282 | 0.7 | 0.541836 |
Target: 5'- uGGCC-GCUGCaC-UGUGCUGGCUCGa -3' miRNA: 3'- gCCGGaCGACGcGcACACGGUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 29422 | 0.69 | 0.608111 |
Target: 5'- gGGCCccggugacaGCUacaaccaGCGCGUGgGCCAGUUCAu -3' miRNA: 3'- gCCGGa--------CGA-------CGCGCACaCGGUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 33935 | 0.68 | 0.638253 |
Target: 5'- uGGUgCUGCUGUGCGgcGUGCgCGGCgagCAg -3' miRNA: 3'- gCCG-GACGACGCGCa-CACG-GUCGa--GU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 40689 | 0.69 | 0.579086 |
Target: 5'- gCGGCgUGCUGUggaacgaaggugaGUGUGUGCUGGCgcgCGg -3' miRNA: 3'- -GCCGgACGACG-------------CGCACACGGUCGa--GU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 42572 | 0.69 | 0.59938 |
Target: 5'- aCGGCUaucGCUGCgGCGUcaGCCAGCUa- -3' miRNA: 3'- -GCCGGa--CGACG-CGCAcaCGGUCGAgu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 53194 | 0.68 | 0.667384 |
Target: 5'- uGGCCaGCgGCGCGcG-GcCCGGCUCGc -3' miRNA: 3'- gCCGGaCGaCGCGCaCaC-GGUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 73014 | 0.66 | 0.77063 |
Target: 5'- uGGCUgacaUGCGCGgugGUGCUGGCUg- -3' miRNA: 3'- gCCGGacg-ACGCGCa--CACGGUCGAgu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 73167 | 0.68 | 0.65769 |
Target: 5'- uCGGCCUGCgaccugGaCGCGgagcugGCCGGCaUCGa -3' miRNA: 3'- -GCCGGACGa-----C-GCGCaca---CGGUCG-AGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 73968 | 0.67 | 0.696282 |
Target: 5'- uGuGCCUGCUGUGCGagcucaUGU-CCuGCUCGu -3' miRNA: 3'- gC-CGGACGACGCGC------ACAcGGuCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 74351 | 0.73 | 0.392194 |
Target: 5'- uGGCgCUGCUGCGCGgcuacgGCCAGUgCGg -3' miRNA: 3'- gCCG-GACGACGCGCaca---CGGUCGaGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 74887 | 0.73 | 0.353531 |
Target: 5'- aCGGCgCUGCUGCGCGagacgGUGgCGGCg-- -3' miRNA: 3'- -GCCG-GACGACGCGCa----CACgGUCGagu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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