Results 1 - 20 of 80 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13992 | 3' | -62.3 | NC_003521.1 | + | 43836 | 0.66 | 0.751483 |
Target: 5'- ---cGCCGCCGGCcgcgGGCCCGUcacAGGc -3' miRNA: 3'- gaccCGGUGGCCGaa--CUGGGCG---UCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 154296 | 0.66 | 0.751483 |
Target: 5'- -gGGGCgGCaCGGCguccggGGCCgaCGCGGGa -3' miRNA: 3'- gaCCCGgUG-GCCGaa----CUGG--GCGUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 129018 | 0.66 | 0.751483 |
Target: 5'- gCUGGcGCCGCCagagGGC---ACCCGCAGa- -3' miRNA: 3'- -GACC-CGGUGG----CCGaacUGGGCGUCca -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 54433 | 0.66 | 0.751483 |
Target: 5'- aCUGcuGCCGCCGcuGCUUGGgCCGaCGGGg -3' miRNA: 3'- -GACc-CGGUGGC--CGAACUgGGC-GUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 86221 | 0.66 | 0.750581 |
Target: 5'- -gGcGGCaCGCCGGCggccuugagcuccUUGACgUGCAGGg -3' miRNA: 3'- gaC-CCG-GUGGCCG-------------AACUGgGCGUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 224534 | 0.66 | 0.74242 |
Target: 5'- -cGGGCCGguaggcaccCCGGC---GCCCaGCAGGa -3' miRNA: 3'- gaCCCGGU---------GGCCGaacUGGG-CGUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 59617 | 0.66 | 0.74242 |
Target: 5'- -gGcGGCC-CCGGCggcgaccgcGGCUCGCAGGc -3' miRNA: 3'- gaC-CCGGuGGCCGaa-------CUGGGCGUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 32987 | 0.66 | 0.74242 |
Target: 5'- -aGGaGCC-CUGGCggaUGAagcCCCGCAGGc -3' miRNA: 3'- gaCC-CGGuGGCCGa--ACU---GGGCGUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 172399 | 0.66 | 0.733272 |
Target: 5'- -aGGGgCGCCGGCggagccguCgCCGCGGGa -3' miRNA: 3'- gaCCCgGUGGCCGaacu----G-GGCGUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 129193 | 0.66 | 0.732353 |
Target: 5'- uUGGGUuuCGCCGGCgguucGACcucgucgCCGCGGGg -3' miRNA: 3'- gACCCG--GUGGCCGaa---CUG-------GGCGUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 88676 | 0.66 | 0.714752 |
Target: 5'- gUGGGCCGgCGGCccGGCUCG-GGGa -3' miRNA: 3'- gACCCGGUgGCCGaaCUGGGCgUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 218034 | 0.66 | 0.714752 |
Target: 5'- uUGGGCgGCggCGGCg---UCCGCAGGUa -3' miRNA: 3'- gACCCGgUG--GCCGaacuGGGCGUCCA- -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 166119 | 0.66 | 0.714752 |
Target: 5'- -cGGGCCugCuucagGGC--GGCCgGCAGGUc -3' miRNA: 3'- gaCCCGGugG-----CCGaaCUGGgCGUCCA- -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 162865 | 0.66 | 0.714752 |
Target: 5'- ---cGCgGCCGGCUccaaGACCCaGCAGGg -3' miRNA: 3'- gaccCGgUGGCCGAa---CUGGG-CGUCCa -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 187589 | 0.66 | 0.714752 |
Target: 5'- -gGGGCUucGCCGGCggucgcuucGCCCGCGGa- -3' miRNA: 3'- gaCCCGG--UGGCCGaac------UGGGCGUCca -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 184638 | 0.66 | 0.714752 |
Target: 5'- cCUGcgccGUCACCGGCUugUGACCCGUAc-- -3' miRNA: 3'- -GACc---CGGUGGCCGA--ACUGGGCGUcca -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 123035 | 0.66 | 0.705397 |
Target: 5'- -gGGGCCGuagaaaCGGCgauaGAUCCGCAGcGUg -3' miRNA: 3'- gaCCCGGUg-----GCCGaa--CUGGGCGUC-CA- -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 60827 | 0.66 | 0.705397 |
Target: 5'- gCUGGccuucaguaGCCACCaGGaCUU--CCCGCAGGUg -3' miRNA: 3'- -GACC---------CGGUGG-CC-GAAcuGGGCGUCCA- -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 206637 | 0.66 | 0.704458 |
Target: 5'- gUGGGCCacgauaaGCUGGUcaagUUGGCCCGCGa-- -3' miRNA: 3'- gACCCGG-------UGGCCG----AACUGGGCGUcca -5' |
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13992 | 3' | -62.3 | NC_003521.1 | + | 56253 | 0.67 | 0.700697 |
Target: 5'- -gGGGCUgaggcagGCCGGCaggcagagguugGACUCGCAGGc -3' miRNA: 3'- gaCCCGG-------UGGCCGaa----------CUGGGCGUCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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