Results 1 - 20 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14148 | 5' | -58.5 | NC_003521.1 | + | 106562 | 1.08 | 0.003168 |
Target: 5'- gCUGCAGAACCUGCACCGGGUCACCCUg -3' miRNA: 3'- -GACGUCUUGGACGUGGCCCAGUGGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 59692 | 0.8 | 0.209016 |
Target: 5'- cCUGgAGAACCUGgACuCGGGUCGCCUg -3' miRNA: 3'- -GACgUCUUGGACgUG-GCCCAGUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 161192 | 0.78 | 0.282425 |
Target: 5'- -aGCAGAcgacGCCgagGCGCCGcGGUCGCCCc -3' miRNA: 3'- gaCGUCU----UGGa--CGUGGC-CCAGUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 6686 | 0.77 | 0.321965 |
Target: 5'- gCUGCAGGGCCUGCGCCcgcgagcgcugcuGGGUCucGCCg- -3' miRNA: 3'- -GACGUCUUGGACGUGG-------------CCCAG--UGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 140627 | 0.76 | 0.38593 |
Target: 5'- cCUGCagcgguacacggccgAGAGCCUGCGCCuGG-CGCCCUa -3' miRNA: 3'- -GACG---------------UCUUGGACGUGGcCCaGUGGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 17640 | 0.73 | 0.494837 |
Target: 5'- gCUGCGGAgcuacguacGCCaGCACCGGGgCACCa- -3' miRNA: 3'- -GACGUCU---------UGGaCGUGGCCCaGUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 120606 | 0.73 | 0.513426 |
Target: 5'- aCUGcCAGGugUUGCGCCuGGUCACCa- -3' miRNA: 3'- -GAC-GUCUugGACGUGGcCCAGUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 65426 | 0.72 | 0.541822 |
Target: 5'- cCUGacccACCUGCGCCGGGUCAugggucCCCg -3' miRNA: 3'- -GACgucuUGGACGUGGCCCAGU------GGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 126963 | 0.71 | 0.639262 |
Target: 5'- -cGCGGAACCcGCGCCGucGUCGCCg- -3' miRNA: 3'- gaCGUCUUGGaCGUGGCc-CAGUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 219234 | 0.71 | 0.639262 |
Target: 5'- uUGCAGGACCcaggucagcaGCGCCGGGUagagcaggGCCCg -3' miRNA: 3'- gACGUCUUGGa---------CGUGGCCCAg-------UGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 205138 | 0.71 | 0.649087 |
Target: 5'- aUGguGAACCaGgGCCGGGUgGCCg- -3' miRNA: 3'- gACguCUUGGaCgUGGCCCAgUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 116024 | 0.7 | 0.668694 |
Target: 5'- -aGCuGGugCUGCcCUGGGUCAUCCc -3' miRNA: 3'- gaCGuCUugGACGuGGCCCAGUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 206237 | 0.7 | 0.67846 |
Target: 5'- uCUGCGcGcGCCUGCAgcCCGGcGUCAgCCUg -3' miRNA: 3'- -GACGU-CuUGGACGU--GGCC-CAGUgGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 72030 | 0.7 | 0.697879 |
Target: 5'- -cGCGuGGCCUGCACCaGGUCcuggugcagACCCUu -3' miRNA: 3'- gaCGUcUUGGACGUGGcCCAG---------UGGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 176446 | 0.7 | 0.706554 |
Target: 5'- uCUGCAGAcgcGCCcGCACCucgggcauguccaGGG-CGCCCa -3' miRNA: 3'- -GACGUCU---UGGaCGUGG-------------CCCaGUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 186317 | 0.7 | 0.707516 |
Target: 5'- gUGCAGAuCCUGguccaCGCCGGGcCGCCg- -3' miRNA: 3'- gACGUCUuGGAC-----GUGGCCCaGUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 22295 | 0.69 | 0.717094 |
Target: 5'- -gGCAGcucuCCUGCACCuucacGGUCugCCUg -3' miRNA: 3'- gaCGUCuu--GGACGUGGc----CCAGugGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 186747 | 0.69 | 0.736041 |
Target: 5'- aUGUAGAACCgguUGCGCCGGa--ACCCc -3' miRNA: 3'- gACGUCUUGG---ACGUGGCCcagUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 66087 | 0.69 | 0.736041 |
Target: 5'- -aGC-GAGcCCUGCGuuGGGcCGCCCUc -3' miRNA: 3'- gaCGuCUU-GGACGUggCCCaGUGGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 109735 | 0.69 | 0.745394 |
Target: 5'- -gGUGGGACUUGUGCaGGGUCACCg- -3' miRNA: 3'- gaCGUCUUGGACGUGgCCCAGUGGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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