Results 1 - 20 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14148 | 5' | -58.5 | NC_003521.1 | + | 234350 | 0.66 | 0.897004 |
Target: 5'- gCUGCcGGACCUGCcucCCGugccGUCAUCCg -3' miRNA: 3'- -GACGuCUUGGACGu--GGCc---CAGUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 229644 | 0.69 | 0.754654 |
Target: 5'- -aGaCAGGugCUGCcucACCGGGaUCAUCCUc -3' miRNA: 3'- gaC-GUCUugGACG---UGGCCC-AGUGGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 226807 | 0.67 | 0.832561 |
Target: 5'- -cGUAGGACCUgacgguacaGCGCCaugGGGUCcucGCCCg -3' miRNA: 3'- gaCGUCUUGGA---------CGUGG---CCCAG---UGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 221899 | 0.67 | 0.832561 |
Target: 5'- -gGCGGAuuGCCgGCGCgCGGcGcCACCCUu -3' miRNA: 3'- gaCGUCU--UGGaCGUG-GCC-CaGUGGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 221344 | 0.66 | 0.870312 |
Target: 5'- -aGCGGGccACCUGCACCuGGcCGCgCa -3' miRNA: 3'- gaCGUCU--UGGACGUGGcCCaGUGgGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 219234 | 0.71 | 0.639262 |
Target: 5'- uUGCAGGACCcaggucagcaGCGCCGGGUagagcaggGCCCg -3' miRNA: 3'- gACGUCUUGGa---------CGUGGCCCAg-------UGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 212510 | 0.68 | 0.781807 |
Target: 5'- -cGCAG-GCC-GCGCCGGGgucgCugCCg -3' miRNA: 3'- gaCGUCuUGGaCGUGGCCCa---GugGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 206237 | 0.7 | 0.67846 |
Target: 5'- uCUGCGcGcGCCUGCAgcCCGGcGUCAgCCUg -3' miRNA: 3'- -GACGU-CuUGGACGU--GGCC-CAGUgGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 205138 | 0.71 | 0.649087 |
Target: 5'- aUGguGAACCaGgGCCGGGUgGCCg- -3' miRNA: 3'- gACguCUUGGaCgUGGCCCAgUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 204127 | 0.66 | 0.890641 |
Target: 5'- cCUGCucGGGCCagGCGCCGGa--GCCCg -3' miRNA: 3'- -GACGu-CUUGGa-CGUGGCCcagUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 201710 | 0.66 | 0.890641 |
Target: 5'- gCUGCAGAcGCCcggacgacUGCGCCaacccuGGGUgAUCCUg -3' miRNA: 3'- -GACGUCU-UGG--------ACGUGG------CCCAgUGGGA- -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 191704 | 0.68 | 0.781807 |
Target: 5'- -cGCAGcAGCg-GCGCCGcGGUCACCa- -3' miRNA: 3'- gaCGUC-UUGgaCGUGGC-CCAGUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 187183 | 0.69 | 0.763816 |
Target: 5'- gUGCGGAgacggcccGCCUGCAUCGGGgaCAUUCg -3' miRNA: 3'- gACGUCU--------UGGACGUGGCCCa-GUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 186747 | 0.69 | 0.736041 |
Target: 5'- aUGUAGAACCgguUGCGCCGGa--ACCCc -3' miRNA: 3'- gACGUCUUGG---ACGUGGCCcagUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 186317 | 0.7 | 0.707516 |
Target: 5'- gUGCAGAuCCUGguccaCGCCGGGcCGCCg- -3' miRNA: 3'- gACGUCUuGGAC-----GUGGCCCaGUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 184626 | 0.67 | 0.855765 |
Target: 5'- gUGCGGuacaGACCUGCGCCG--UCACCg- -3' miRNA: 3'- gACGUC----UUGGACGUGGCccAGUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 183547 | 0.67 | 0.832561 |
Target: 5'- -cGCAccGACCUGCuGCUGGGUCGCUUc -3' miRNA: 3'- gaCGUc-UUGGACG-UGGCCCAGUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 178794 | 0.66 | 0.88407 |
Target: 5'- uUGCAGGACggGCccCCGGG-CACCUc -3' miRNA: 3'- gACGUCUUGgaCGu-GGCCCaGUGGGa -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 177545 | 0.66 | 0.877291 |
Target: 5'- -aGCAGcugCUGCACCcGGUCGCCg- -3' miRNA: 3'- gaCGUCuugGACGUGGcCCAGUGGga -5' |
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14148 | 5' | -58.5 | NC_003521.1 | + | 176446 | 0.7 | 0.706554 |
Target: 5'- uCUGCAGAcgcGCCcGCACCucgggcauguccaGGG-CGCCCa -3' miRNA: 3'- -GACGUCU---UGGaCGUGG-------------CCCaGUGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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