Results 1 - 20 of 103 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14282 | 5' | -56.1 | NC_003521.1 | + | 3657 | 0.66 | 0.956089 |
Target: 5'- gCCGCCGUCugccucgGCGG-CCGGGacccuCGUCGg -3' miRNA: 3'- aGGUGGCAG-------UGCCaGGCUCgu---GUAGU- -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 4363 | 0.69 | 0.876982 |
Target: 5'- gUUCGCCGcUUGCGG-CCGAGCAgGUUc -3' miRNA: 3'- -AGGUGGC-AGUGCCaGGCUCGUgUAGu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 5707 | 0.68 | 0.91693 |
Target: 5'- -gCGCCGUCaccgggguuauguguACGGcCCGGGCACGgcgCGu -3' miRNA: 3'- agGUGGCAG---------------UGCCaGGCUCGUGUa--GU- -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 8558 | 1.06 | 0.008289 |
Target: 5'- aUCCACCGUCACGGUCCGAGCACAUCc -3' miRNA: 3'- -AGGUGGCAGUGCCAGGCUCGUGUAGu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 13473 | 0.71 | 0.78982 |
Target: 5'- -gCGCCGUCgACGGUCUGGGCgACGg-- -3' miRNA: 3'- agGUGGCAG-UGCCAGGCUCG-UGUagu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 14115 | 0.72 | 0.70627 |
Target: 5'- gCCugUGUCugGGUCCGccgaagggcuGGCACGUg- -3' miRNA: 3'- aGGugGCAGugCCAGGC----------UCGUGUAgu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 14531 | 0.71 | 0.775579 |
Target: 5'- aCCACgcccgagggcucguaCGcCACGG-CCGAGCGCAUCc -3' miRNA: 3'- aGGUG---------------GCaGUGCCaGGCUCGUGUAGu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 18539 | 0.66 | 0.963367 |
Target: 5'- -aCGCUGgCACGGggcUCUGGGCACcAUCAc -3' miRNA: 3'- agGUGGCaGUGCC---AGGCUCGUG-UAGU- -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 21095 | 0.66 | 0.960015 |
Target: 5'- cCCGCgaCGgCGCGGUCCcuccgcGCACGUCAc -3' miRNA: 3'- aGGUG--GCaGUGCCAGGcu----CGUGUAGU- -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 27555 | 0.7 | 0.806288 |
Target: 5'- cUCCACCGUC---GUCCGGGCggucgacGCGUCGg -3' miRNA: 3'- -AGGUGGCAGugcCAGGCUCG-------UGUAGU- -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 31270 | 0.71 | 0.770158 |
Target: 5'- gCCACCGUCGCcgccaccccgCCGGGCGCAUa- -3' miRNA: 3'- aGGUGGCAGUGcca-------GGCUCGUGUAgu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 31823 | 0.69 | 0.883797 |
Target: 5'- -gCGCCGUCuCGGcCCGGGCGCc--- -3' miRNA: 3'- agGUGGCAGuGCCaGGCUCGUGuagu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 36938 | 0.73 | 0.647566 |
Target: 5'- aCUGCCGUCAUGGgcCCGAcGCugGUCGu -3' miRNA: 3'- aGGUGGCAGUGCCa-GGCU-CGugUAGU- -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 40485 | 0.69 | 0.876982 |
Target: 5'- gUCCGCggcgCGcCACGGcgCCGcAGCGCAUCu -3' miRNA: 3'- -AGGUG----GCaGUGCCa-GGC-UCGUGUAGu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 45644 | 0.69 | 0.862745 |
Target: 5'- gCCGCCGgcuccgCGgGGUCCGGcGCACAc-- -3' miRNA: 3'- aGGUGGCa-----GUgCCAGGCU-CGUGUagu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 47437 | 0.7 | 0.839955 |
Target: 5'- gUCCACCGagAaaccCGcGUCCaaGAGCACGUCGg -3' miRNA: 3'- -AGGUGGCagU----GC-CAGG--CUCGUGUAGU- -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 47949 | 0.72 | 0.753655 |
Target: 5'- aCCGCCGUCGCGcUCgGAaGCACAagUCGc -3' miRNA: 3'- aGGUGGCAGUGCcAGgCU-CGUGU--AGU- -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 48109 | 0.7 | 0.839955 |
Target: 5'- aCCACCGcuaUCGCGGgaCGAGCGCGa-- -3' miRNA: 3'- aGGUGGC---AGUGCCagGCUCGUGUagu -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 48597 | 0.69 | 0.869963 |
Target: 5'- cCCGCUGUCAC--UCCGuGCACcGUCAg -3' miRNA: 3'- aGGUGGCAGUGccAGGCuCGUG-UAGU- -5' |
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14282 | 5' | -56.1 | NC_003521.1 | + | 50193 | 0.66 | 0.956089 |
Target: 5'- cUCCGCCuccuGUC-CGGUCCgcccgaaGAGCGCgaagGUCAg -3' miRNA: 3'- -AGGUGG----CAGuGCCAGG-------CUCGUG----UAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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