Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14659 | 3' | -54.4 | NC_003525.1 | + | 4721 | 0.7 | 0.52986 |
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Target: 5'- aAGCAcgaaGCAG-ACCAgcGCGGGugCGCCGg -3' miRNA: 3'- gUCGU----UGUCgUGGU--UGUCCugGUGGU- -5' |
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| 14659 | 3' | -54.4 | NC_003525.1 | + | 45847 | 0.71 | 0.436045 |
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Target: 5'- gAGCGGCAGCAaguggggaaCAGCAGaagaccuGACCGCCGc -3' miRNA: 3'- gUCGUUGUCGUg--------GUUGUC-------CUGGUGGU- -5' |
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| 14659 | 3' | -54.4 | NC_003525.1 | + | 43645 | 0.72 | 0.427284 |
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Target: 5'- uCGGCgAACAGCGCCccguguGGCAGGGCaauCCAg -3' miRNA: 3'- -GUCG-UUGUCGUGG------UUGUCCUGgu-GGU- -5' |
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| 14659 | 3' | -54.4 | NC_003525.1 | + | 55809 | 0.72 | 0.417673 |
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Target: 5'- aGGgAACAcCGCCAGCAGaGCCACCGa -3' miRNA: 3'- gUCgUUGUcGUGGUUGUCcUGGUGGU- -5' |
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| 14659 | 3' | -54.4 | NC_003525.1 | + | 60035 | 0.72 | 0.389648 |
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Target: 5'- aCAGCAGUAGCACCAA-AGGAaaCCAUCAc -3' miRNA: 3'- -GUCGUUGUCGUGGUUgUCCU--GGUGGU- -5' |
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| 14659 | 3' | -54.4 | NC_003525.1 | + | 18673 | 0.74 | 0.313284 |
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Target: 5'- gAGCGAUAGCcuuAUCAACGGGuAUCGCCAg -3' miRNA: 3'- gUCGUUGUCG---UGGUUGUCC-UGGUGGU- -5' |
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| 14659 | 3' | -54.4 | NC_003525.1 | + | 8391 | 0.74 | 0.305534 |
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Target: 5'- aCAGUgcaaaGACGGCaACCAGCAGGGCgGCUg -3' miRNA: 3'- -GUCG-----UUGUCG-UGGUUGUCCUGgUGGu -5' |
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| 14659 | 3' | -54.4 | NC_003525.1 | + | 21584 | 0.78 | 0.170686 |
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Target: 5'- -cGCGACAGCACCggUacgugaAGGGCUACCGg -3' miRNA: 3'- guCGUUGUCGUGGuuG------UCCUGGUGGU- -5' |
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| 14659 | 3' | -54.4 | NC_003525.1 | + | 17810 | 1.09 | 0.001221 |
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Target: 5'- cCAGCAACAGCACCAACAGGACCACCAa -3' miRNA: 3'- -GUCGUUGUCGUGGUUGUCCUGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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