Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1524 | 3' | -55.7 | NC_001335.1 | + | 10898 | 0.68 | 0.523011 |
Target: 5'- cGCCAGGAgGCUGCagCCgCUCGggUGgCGa -3' miRNA: 3'- -CGGUUCU-CGAUG--GGaGGGCuuACgGC- -5' |
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1524 | 3' | -55.7 | NC_001335.1 | + | 24146 | 0.71 | 0.367615 |
Target: 5'- cGCCAGGAGCauUGCCgUUCCCGccaGCUGa -3' miRNA: 3'- -CGGUUCUCG--AUGG-GAGGGCuuaCGGC- -5' |
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1524 | 3' | -55.7 | NC_001335.1 | + | 11418 | 0.72 | 0.318071 |
Target: 5'- uUCGAGGGCUACCUcgagCCCGAGcagGCCc -3' miRNA: 3'- cGGUUCUCGAUGGGa---GGGCUUa--CGGc -5' |
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1524 | 3' | -55.7 | NC_001335.1 | + | 22675 | 0.74 | 0.24066 |
Target: 5'- gGCCGAGGGUacUACCgUgCCGAcgGCCa -3' miRNA: 3'- -CGGUUCUCG--AUGGgAgGGCUuaCGGc -5' |
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1524 | 3' | -55.7 | NC_001335.1 | + | 51161 | 1.12 | 0.000556 |
Target: 5'- cGCCAAGAGCUACCCUCCCGAAUGCCGa -3' miRNA: 3'- -CGGUUCUCGAUGGGAGGGCUUACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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