Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16250 | 3' | -56 | NC_004084.1 | + | 48243 | 0.66 | 0.773191 |
Target: 5'- aCGUCGaggacugggauUC-CGAGaaCCUCGaGCCGGa -3' miRNA: 3'- gGCAGU-----------AGaGCUCaaGGAGC-CGGCCc -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 911 | 0.66 | 0.773191 |
Target: 5'- gCGUCGUCg-GAGUUCgCUgCGGCCu-- -3' miRNA: 3'- gGCAGUAGagCUCAAG-GA-GCCGGccc -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 21552 | 0.66 | 0.773191 |
Target: 5'- uCCGUCggCUUGAG-UCCacgCuGCCGGa -3' miRNA: 3'- -GGCAGuaGAGCUCaAGGa--GcCGGCCc -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 3100 | 0.66 | 0.773191 |
Target: 5'- aCCGUCG-CU-GAGUUCCUCGaugagaGGGa -3' miRNA: 3'- -GGCAGUaGAgCUCAAGGAGCcgg---CCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 38191 | 0.66 | 0.76338 |
Target: 5'- gCGUCAUCUCGAaugacgucgacGUg-CUUGcGCCGGa -3' miRNA: 3'- gGCAGUAGAGCU-----------CAagGAGC-CGGCCc -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 41948 | 0.66 | 0.76338 |
Target: 5'- gUCGUC-UCUCGAGgcCCU--GCCGGa -3' miRNA: 3'- -GGCAGuAGAGCUCaaGGAgcCGGCCc -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 35877 | 0.66 | 0.753444 |
Target: 5'- aCCG-CGUgaUCGGucgUCUUCGGCCGGa -3' miRNA: 3'- -GGCaGUAg-AGCUca-AGGAGCCGGCCc -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 1999 | 0.66 | 0.753444 |
Target: 5'- aCGUCGUCgaggacgcgaUCGAGUUCgaCUCccaGCUGGGc -3' miRNA: 3'- gGCAGUAG----------AGCUCAAG--GAGc--CGGCCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 52626 | 0.66 | 0.753444 |
Target: 5'- cCCGgCGUCggauaccagggUGAGUgCCUCGGCUGcGGc -3' miRNA: 3'- -GGCaGUAGa----------GCUCAaGGAGCCGGC-CC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 10704 | 0.66 | 0.743391 |
Target: 5'- gCCGgug-CUCG-GUUCuucgCUCGGCuCGGGg -3' miRNA: 3'- -GGCaguaGAGCuCAAG----GAGCCG-GCCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 43735 | 0.66 | 0.743391 |
Target: 5'- -aGUUGggccaaUCGAGgaCC-CGGCCGGGa -3' miRNA: 3'- ggCAGUag----AGCUCaaGGaGCCGGCCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 21147 | 0.66 | 0.74238 |
Target: 5'- aCGUCcuggauuGUCgagagCGAG-UCCUCGacgagcGCCGGGa -3' miRNA: 3'- gGCAG-------UAGa----GCUCaAGGAGC------CGGCCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 55360 | 0.67 | 0.712644 |
Target: 5'- cCCGUCGa--CGAGUUCCUCGa--GGGu -3' miRNA: 3'- -GGCAGUagaGCUCAAGGAGCcggCCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 52686 | 0.67 | 0.712644 |
Target: 5'- gCGaUCAUCccgaUCGAGgaCCUCGGCCc-- -3' miRNA: 3'- gGC-AGUAG----AGCUCaaGGAGCCGGccc -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 54930 | 0.67 | 0.702232 |
Target: 5'- gCCGUCucgaUCGGGaUCCUCcGCCucGGGa -3' miRNA: 3'- -GGCAGuag-AGCUCaAGGAGcCGG--CCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 50955 | 0.67 | 0.691757 |
Target: 5'- gCCGUCGccgCUCGAGUcgCCgUCGGacuCCGaGGu -3' miRNA: 3'- -GGCAGUa--GAGCUCAa-GG-AGCC---GGC-CC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 14658 | 0.67 | 0.691757 |
Target: 5'- cCCaGUCGaugCccgCGAGcgUCC-CGGCCGGGu -3' miRNA: 3'- -GG-CAGUa--Ga--GCUCa-AGGaGCCGGCCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 35263 | 0.67 | 0.691757 |
Target: 5'- uCCGUCc---CGAGggggaagUCCUCGGCgcUGGGg -3' miRNA: 3'- -GGCAGuagaGCUCa------AGGAGCCG--GCCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 35187 | 0.67 | 0.691757 |
Target: 5'- uCCGggCcgCUCGAGcUCCUCcgaaucGUCGGGa -3' miRNA: 3'- -GGCa-GuaGAGCUCaAGGAGc-----CGGCCC- -5' |
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16250 | 3' | -56 | NC_004084.1 | + | 35668 | 0.67 | 0.681228 |
Target: 5'- aCCGUC-UCgUCGAugUCCUCGuaccGCUGGGc -3' miRNA: 3'- -GGCAGuAG-AGCUcaAGGAGC----CGGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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