Results 1 - 20 of 74 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16253 | 3' | -56.4 | NC_004084.1 | + | 30027 | 0.66 | 0.715444 |
Target: 5'- --gCGCCGAUCGAggucucgcgGgGGCCGCUcgCg -3' miRNA: 3'- ccaGUGGCUAGCU---------CgUCGGCGGuaGg -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 36832 | 0.66 | 0.715444 |
Target: 5'- --aCGCCGAUCGAaCgAGCCGCgAaacggaugcUCCg -3' miRNA: 3'- ccaGUGGCUAGCUcG-UCGGCGgU---------AGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 4834 | 0.66 | 0.715444 |
Target: 5'- -cUCGCCGAUCGucGCgAGCa--CAUCCg -3' miRNA: 3'- ccAGUGGCUAGCu-CG-UCGgcgGUAGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 21059 | 0.66 | 0.715444 |
Target: 5'- cGGcCACCGcagugcuUCGAGCuGGgCGUCGUCg -3' miRNA: 3'- -CCaGUGGCu------AGCUCG-UCgGCGGUAGg -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 15827 | 0.66 | 0.714397 |
Target: 5'- cGGcUC-CUGGUCGuuguagcacgaguGGCAGCuCGCCccAUCCa -3' miRNA: 3'- -CC-AGuGGCUAGC-------------UCGUCG-GCGG--UAGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 17830 | 0.66 | 0.704935 |
Target: 5'- aGGUCcgagaaGCCGAgcUCGAGaCGGCCGagaUGUCg -3' miRNA: 3'- -CCAG------UGGCU--AGCUC-GUCGGCg--GUAGg -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 11046 | 0.66 | 0.704935 |
Target: 5'- cGUCGacgacgcggaCGAagGGGCGGCCGCCGa-- -3' miRNA: 3'- cCAGUg---------GCUagCUCGUCGGCGGUagg -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 51157 | 0.66 | 0.704935 |
Target: 5'- aGGagG-CGAUCGAGaCGGUCGCCGagCa -3' miRNA: 3'- -CCagUgGCUAGCUC-GUCGGCGGUagG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 46874 | 0.66 | 0.694358 |
Target: 5'- cGUUugCG-UCG-GCuGCCGCCAcgCCc -3' miRNA: 3'- cCAGugGCuAGCuCGuCGGCGGUa-GG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 10670 | 0.66 | 0.694358 |
Target: 5'- cGUUugCGAUCGccgGGCAGC-GaCCGUUCg -3' miRNA: 3'- cCAGugGCUAGC---UCGUCGgC-GGUAGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 14870 | 0.66 | 0.683723 |
Target: 5'- cGGcCGCCGGUCucGguGCCGUgcuuCGUCa -3' miRNA: 3'- -CCaGUGGCUAGcuCguCGGCG----GUAGg -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 54567 | 0.67 | 0.662322 |
Target: 5'- uGGaCACCGAgguucCGGuGgAGCCGUCGUCg -3' miRNA: 3'- -CCaGUGGCUa----GCU-CgUCGGCGGUAGg -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 16124 | 0.67 | 0.640813 |
Target: 5'- cGUCGCCGAUCGugaacGGguGaCCGCgGUg- -3' miRNA: 3'- cCAGUGGCUAGC-----UCguC-GGCGgUAgg -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 10905 | 0.67 | 0.640813 |
Target: 5'- gGGUacucCCGAUCGGGgAGuUCGUCGUCUu -3' miRNA: 3'- -CCAgu--GGCUAGCUCgUC-GGCGGUAGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 51215 | 0.67 | 0.640813 |
Target: 5'- gGGcUACUGGgcguggugcgUCGAGCAGCuCGaCGUCCg -3' miRNA: 3'- -CCaGUGGCU----------AGCUCGUCG-GCgGUAGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 39077 | 0.67 | 0.638659 |
Target: 5'- aGGUCGCUGucgcgcuugagUGAGaCAGUCGCCcgUCg -3' miRNA: 3'- -CCAGUGGCua---------GCUC-GUCGGCGGuaGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 16621 | 0.67 | 0.631119 |
Target: 5'- cGGUCuguggaagACCGAUCuuuucggggcggguGAcGCAGCCGCUggagugcuugguagaAUCCg -3' miRNA: 3'- -CCAG--------UGGCUAG--------------CU-CGUCGGCGG---------------UAGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 49455 | 0.67 | 0.619274 |
Target: 5'- cGUCGCCGccGUCuucGUcGCCGCUGUCCu -3' miRNA: 3'- cCAGUGGC--UAGcu-CGuCGGCGGUAGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 20202 | 0.67 | 0.619274 |
Target: 5'- aGUCGCCGcAUCGAacgacagcGCGGCCGUUuuggaccucUCCg -3' miRNA: 3'- cCAGUGGC-UAGCU--------CGUCGGCGGu--------AGG- -5' |
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16253 | 3' | -56.4 | NC_004084.1 | + | 42259 | 0.67 | 0.619274 |
Target: 5'- uGGUCGagUGAugcUCGAGCAGCgCGaCCA-CCg -3' miRNA: 3'- -CCAGUg-GCU---AGCUCGUCG-GC-GGUaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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