Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16635 | 3' | -54.5 | NC_004156.1 | + | 58542 | 0.66 | 0.979747 |
Target: 5'- nCGaAAGGCuGAuGCUGAAGCaGCCGa -3' miRNA: 3'- -GCaUUUCGcCUcCGACUUCGaCGGCg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 57946 | 0.66 | 0.977488 |
Target: 5'- aCGUuuGGCGGcAGcuucagccuccGCuuUGAguuuGGCUGCCGCu -3' miRNA: 3'- -GCAuuUCGCC-UC-----------CG--ACU----UCGACGGCG- -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 196340 | 0.66 | 0.975048 |
Target: 5'- --aGAGGCGGAGuCUGA-GCcgGCCGa -3' miRNA: 3'- gcaUUUCGCCUCcGACUuCGa-CGGCg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 55581 | 0.66 | 0.972422 |
Target: 5'- --gAAAGUGGAcuagagguuuauGGCU--AGCUGCUGCc -3' miRNA: 3'- gcaUUUCGCCU------------CCGAcuUCGACGGCG- -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 57532 | 0.66 | 0.972422 |
Target: 5'- aCGcAAAGCcGAuGCUGAAGCaGCCGa -3' miRNA: 3'- -GCaUUUCGcCUcCGACUUCGaCGGCg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 57613 | 0.66 | 0.972422 |
Target: 5'- aCGcAAAGCcGAuGCUGAAGCaGCCGa -3' miRNA: 3'- -GCaUUUCGcCUcCGACUUCGaCGGCg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 149432 | 0.66 | 0.968716 |
Target: 5'- ------cCGGAGGUgugguagaguacgaUGGAGCUGCCGa -3' miRNA: 3'- gcauuucGCCUCCG--------------ACUUCGACGGCg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 41313 | 0.67 | 0.963353 |
Target: 5'- aGUGGAGgauuCGGAGGCgguuucgGAGGCucaggaggaggUGCCGg -3' miRNA: 3'- gCAUUUC----GCCUCCGa------CUUCG-----------ACGGCg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 3281 | 0.68 | 0.939409 |
Target: 5'- aGUGAAGcCGGuGGCgguAGUagaauUGCCGCc -3' miRNA: 3'- gCAUUUC-GCCuCCGacuUCG-----ACGGCG- -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 58476 | 0.71 | 0.827407 |
Target: 5'- aCGUAAAGCcGAuGCUGAAGCaGCaaaGCg -3' miRNA: 3'- -GCAUUUCGcCUcCGACUUCGaCGg--CG- -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 58569 | 0.71 | 0.827407 |
Target: 5'- aCGUAAAGCcGAuGCUGAAGCaGCaaaGCg -3' miRNA: 3'- -GCAUUUCGcCUcCGACUUCGaCGg--CG- -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 58623 | 0.71 | 0.827407 |
Target: 5'- aCGUAAAGCcGAuGCUGAAGCaGCaaaGCg -3' miRNA: 3'- -GCAUUUCGcCUcCGACUUCGaCGg--CG- -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 85642 | 0.74 | 0.647243 |
Target: 5'- aCGUAAAGCgGGAGGCuccuuUGAAGCgGuaGCg -3' miRNA: 3'- -GCAUUUCG-CCUCCG-----ACUUCGaCggCG- -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 58509 | 0.75 | 0.637132 |
Target: 5'- aCGUAAAGCcGAuGCUGAAGCaGCCGa -3' miRNA: 3'- -GCAUUUCGcCUcCGACUUCGaCGGCg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 58084 | 0.76 | 0.576649 |
Target: 5'- aCGUAAAGCuGAGaUUGAAGCaGCCGCc -3' miRNA: 3'- -GCAUUUCGcCUCcGACUUCGaCGGCG- -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 58294 | 0.77 | 0.51753 |
Target: 5'- aCGUAAAGCcGAGGCUGAAGCaGCa-- -3' miRNA: 3'- -GCAUUUCGcCUCCGACUUCGaCGgcg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 57964 | 0.78 | 0.430414 |
Target: 5'- aCGUAAAGCcGAGGCUGAAGCagcaaaacgaaagGCCGa -3' miRNA: 3'- -GCAUUUCGcCUCCGACUUCGa------------CGGCg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 57907 | 0.79 | 0.413 |
Target: 5'- aCGUAAAGCuGAGGCUGAGGCagcaaaacgaaagGCCGa -3' miRNA: 3'- -GCAUUUCGcCUCCGACUUCGa------------CGGCg -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 58237 | 0.82 | 0.299944 |
Target: 5'- aCGUAAAGCcGAGGCUGAAGCgGCaGCc -3' miRNA: 3'- -GCAUUUCGcCUCCGACUUCGaCGgCG- -5' |
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16635 | 3' | -54.5 | NC_004156.1 | + | 58177 | 0.82 | 0.299944 |
Target: 5'- aCGUAAAGCcGAGGCUGAAGCgGCaGCc -3' miRNA: 3'- -GCAUUUCGcCUCCGACUUCGaCGgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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