Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16687 | 5' | -53.2 | NC_004156.1 | + | 112903 | 0.69 | 0.942549 |
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Target: 5'- ---aGCGgGCCACUACAUCU-GCCGa -3' miRNA: 3'- aguaCGUgUGGUGAUGUGGAgUGGCa -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 15935 | 0.69 | 0.937872 |
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Target: 5'- cCAUGCGUACCGagGCGCC-CACCGg -3' miRNA: 3'- aGUACGUGUGGUgaUGUGGaGUGGCa -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 137843 | 0.69 | 0.927808 |
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Target: 5'- aCAUGCAUACaCAaacacauuggcUUACACCUCACUa- -3' miRNA: 3'- aGUACGUGUG-GU-----------GAUGUGGAGUGGca -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 99224 | 0.71 | 0.89126 |
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Target: 5'- cCAUGCACugCACUcaaccauACACUgaaUCACCa- -3' miRNA: 3'- aGUACGUGugGUGA-------UGUGG---AGUGGca -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 215276 | 0.71 | 0.863444 |
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Target: 5'- cUC-UGCACAaaaaCACUACAUC-CACCGUu -3' miRNA: 3'- -AGuACGUGUg---GUGAUGUGGaGUGGCA- -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 151295 | 0.72 | 0.839883 |
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Target: 5'- aUCGuUGCGCACUcggacCACCUCACCGUu -3' miRNA: 3'- -AGU-ACGUGUGGugau-GUGGAGUGGCA- -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 193608 | 0.72 | 0.823223 |
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Target: 5'- aUCAUGCACAgUACUACGCugcgauCUUugCGUg -3' miRNA: 3'- -AGUACGUGUgGUGAUGUG------GAGugGCA- -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 144581 | 1.09 | 0.007889 |
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Target: 5'- uUCAUGCACACCACUACACCUCACCGUa -3' miRNA: 3'- -AGUACGUGUGGUGAUGUGGAGUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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