Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16692 | 5' | -49 | NC_004156.1 | + | 71021 | 0.66 | 0.999519 |
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Target: 5'- -uUCAAcUugGGcgaAUGCAUGCACaUUGGc -3' miRNA: 3'- auAGUUuGugCC---UACGUACGUG-AGCC- -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 32060 | 0.66 | 0.999614 |
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Target: 5'- gAUCAAAgGcCGGG-GCuagGUACUCGGu -3' miRNA: 3'- aUAGUUUgU-GCCUaCGua-CGUGAGCC- -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 130916 | 0.66 | 0.999614 |
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Target: 5'- aAUCAAACugGGccaUGUGCACUUGa -3' miRNA: 3'- aUAGUUUGugCCuacGUACGUGAGCc -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 87115 | 0.66 | 0.999686 |
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Target: 5'- cUAUCAAcgccuACugGuauguuuGAUGCAacGCACUCGGu -3' miRNA: 3'- -AUAGUU-----UGugC-------CUACGUa-CGUGAGCC- -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 212104 | 0.66 | 0.999693 |
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Target: 5'- --cCGGAUACGGucUGCAcuUGCACaCGGu -3' miRNA: 3'- auaGUUUGUGCCu-ACGU--ACGUGaGCC- -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 95828 | 0.66 | 0.999757 |
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Target: 5'- cGUCAaccuGACugGGGUGCcuAUGCAagUGGa -3' miRNA: 3'- aUAGU----UUGugCCUACG--UACGUgaGCC- -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 137097 | 0.66 | 0.999805 |
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Target: 5'- --cCGAGCGCGGuccucauguacauGUGC-UGUACUCGu -3' miRNA: 3'- auaGUUUGUGCC-------------UACGuACGUGAGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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