Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16692 | 5' | -49 | NC_004156.1 | + | 187983 | 0.68 | 0.998075 |
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Target: 5'- gGUCGAgcuGCAC-GAUGCuUGCACUCu- -3' miRNA: 3'- aUAGUU---UGUGcCUACGuACGUGAGcc -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 193912 | 0.67 | 0.999403 |
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Target: 5'- cAUC-GugAUGGuUGCGUGCACUCc- -3' miRNA: 3'- aUAGuUugUGCCuACGUACGUGAGcc -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 203365 | 0.73 | 0.953033 |
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Target: 5'- aAUCuugcAGCACGGAUGCcgaaccUGUACUUGGu -3' miRNA: 3'- aUAGu---UUGUGCCUACGu-----ACGUGAGCC- -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 205613 | 0.67 | 0.9989 |
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Target: 5'- gAUCGGuACACGGGUGCAcagauggagGCACgaagCGa -3' miRNA: 3'- aUAGUU-UGUGCCUACGUa--------CGUGa---GCc -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 212104 | 0.66 | 0.999693 |
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Target: 5'- --cCGGAUACGGucUGCAcuUGCACaCGGu -3' miRNA: 3'- auaGUUUGUGCCu-ACGU--ACGUGaGCC- -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 218488 | 0.67 | 0.998668 |
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Target: 5'- cAUCAA--GCGGAuuaaagUGCAU-CACUCGGu -3' miRNA: 3'- aUAGUUugUGCCU------ACGUAcGUGAGCC- -5' |
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| 16692 | 5' | -49 | NC_004156.1 | + | 226223 | 0.69 | 0.994012 |
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Target: 5'- ---uGAACaaACGGAUGCAaaaUGgACUCGGc -3' miRNA: 3'- auagUUUG--UGCCUACGU---ACgUGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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