Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17604 | 3' | -46.3 | NC_004466.2 | + | 29515 | 0.68 | 0.971649 |
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Target: 5'- aGUGuC-CCAUAGGACGGuCAGGCCa- -3' miRNA: 3'- cCAUuGuGGUAUUCUGUU-GUCUGGcc -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 4498 | 0.68 | 0.971649 |
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Target: 5'- aGG-GACGuCCAaGAGACcgUAGACUGGa -3' miRNA: 3'- -CCaUUGU-GGUaUUCUGuuGUCUGGCC- -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 13985 | 0.67 | 0.98663 |
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Target: 5'- uGGUAACGCCAUGcaGGuuGAU--GCUGGa -3' miRNA: 3'- -CCAUUGUGGUAU--UCugUUGucUGGCC- -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 22353 | 0.67 | 0.98663 |
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Target: 5'- uGGUGACGCUGUAGGcgGCGAagaGGAUUGa -3' miRNA: 3'- -CCAUUGUGGUAUUC--UGUUg--UCUGGCc -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 33590 | 0.66 | 0.991332 |
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Target: 5'- cGU-ACACCGUAAGAUcACGGuuCCGu -3' miRNA: 3'- cCAuUGUGGUAUUCUGuUGUCu-GGCc -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 40151 | 0.81 | 0.418676 |
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Target: 5'- cGGUGACGCCAgaagugaGGGGCGACAuGCUGGg -3' miRNA: 3'- -CCAUUGUGGUa------UUCUGUUGUcUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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