Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17604 | 3' | -46.3 | NC_004466.2 | + | 11338 | 0.69 | 0.964663 |
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Target: 5'- uGGUAGgGuCCGUuGGAuccCAAgAGGCCGGg -3' miRNA: 3'- -CCAUUgU-GGUAuUCU---GUUgUCUGGCC- -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 1024 | 0.7 | 0.93101 |
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Target: 5'- --gGAUACCAUAAGGC--UAGuCCGGg -3' miRNA: 3'- ccaUUGUGGUAUUCUGuuGUCuGGCC- -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 17049 | 0.77 | 0.630096 |
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Target: 5'- aGGccAGCACCAccUAGGACAGCAGuCCaGGg -3' miRNA: 3'- -CCa-UUGUGGU--AUUCUGUUGUCuGG-CC- -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 3123 | 0.81 | 0.398854 |
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Target: 5'- gGGUAACgACCAgUAAGAUGACAGGCCa- -3' miRNA: 3'- -CCAUUG-UGGU-AUUCUGUUGUCUGGcc -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 15218 | 1.14 | 0.004108 |
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Target: 5'- gGGUAACACCAUAAGACAACAGACCGGc -3' miRNA: 3'- -CCAUUGUGGUAUUCUGUUGUCUGGCC- -5' |
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| 17604 | 3' | -46.3 | NC_004466.2 | + | 88 | 0.66 | 0.992214 |
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Target: 5'- aGGacAgACCAUAAcGACGACacuaacgcggaaagGGGCCGGc -3' miRNA: 3'- -CCauUgUGGUAUU-CUGUUG--------------UCUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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