Results 1 - 20 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18048 | 3' | -53.9 | NC_004680.1 | + | 22847 | 0.66 | 0.84841 |
Target: 5'- cCCGgggCGGACgCCGaCAaacuCGCCGAACCa -3' miRNA: 3'- uGGCa--GUUUG-GGCaGU----GUGGCUUGGc -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 46025 | 0.66 | 0.84841 |
Target: 5'- gGCCGc----CCCGUCAcCGCCGAauucaccaccACCGa -3' miRNA: 3'- -UGGCaguuuGGGCAGU-GUGGCU----------UGGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 20752 | 0.66 | 0.84841 |
Target: 5'- cGCCGaUCGGGCCUGUUcCGCC--GCCa -3' miRNA: 3'- -UGGC-AGUUUGGGCAGuGUGGcuUGGc -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 31625 | 0.66 | 0.845851 |
Target: 5'- cGCCGUCGgcgaacaccucuccAACCUGUUucccguccgGCACUGGcacACCGg -3' miRNA: 3'- -UGGCAGU--------------UUGGGCAG---------UGUGGCU---UGGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 17415 | 0.66 | 0.839805 |
Target: 5'- -aCGUCAccGACCCcgccugacguUCACACCGAaaauagaucgucGCCGg -3' miRNA: 3'- ugGCAGU--UUGGGc---------AGUGUGGCU------------UGGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 41742 | 0.66 | 0.839805 |
Target: 5'- cACCGcCGAccccGCCCGcCGCaucuGCUGGACCu -3' miRNA: 3'- -UGGCaGUU----UGGGCaGUG----UGGCUUGGc -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 20590 | 0.66 | 0.838932 |
Target: 5'- gGCCGUCAAAcuucuauCCCG--GCACCGAuuauCUGu -3' miRNA: 3'- -UGGCAGUUU-------GGGCagUGUGGCUu---GGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 23864 | 0.66 | 0.830984 |
Target: 5'- cGCCGUCAcAUCgGUgACAUCG-GCCa -3' miRNA: 3'- -UGGCAGUuUGGgCAgUGUGGCuUGGc -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 24144 | 0.66 | 0.830984 |
Target: 5'- cACCGUCuuGAAUcuucgCCGaCGuCACCGAACCa -3' miRNA: 3'- -UGGCAG--UUUG-----GGCaGU-GUGGCUUGGc -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 18950 | 0.66 | 0.830984 |
Target: 5'- uCgGUgAucCCCGcCACGCCGuAGCCGa -3' miRNA: 3'- uGgCAgUuuGGGCaGUGUGGC-UUGGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 45268 | 0.66 | 0.830984 |
Target: 5'- -aCGUUGGugUCGUCGCGgCGAgaGCCGc -3' miRNA: 3'- ugGCAGUUugGGCAGUGUgGCU--UGGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 42709 | 0.66 | 0.821958 |
Target: 5'- uGCaGUCGAACCgCGUUGCGCCGcaaguuccCCGg -3' miRNA: 3'- -UGgCAGUUUGG-GCAGUGUGGCuu------GGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 15863 | 0.66 | 0.821958 |
Target: 5'- aGCCGUac-GCCuauuuCGUgGCGCCuGAGCCGg -3' miRNA: 3'- -UGGCAguuUGG-----GCAgUGUGG-CUUGGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 10984 | 0.66 | 0.821958 |
Target: 5'- cGCCGUCGGGCUgGUC---CUGAAUCGg -3' miRNA: 3'- -UGGCAGUUUGGgCAGuguGGCUUGGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 1716 | 0.66 | 0.819211 |
Target: 5'- aGCCGUCGcaucucuccaccgcGACCCGUCAaACCcAGCa- -3' miRNA: 3'- -UGGCAGU--------------UUGGGCAGUgUGGcUUGgc -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 57340 | 0.66 | 0.812735 |
Target: 5'- gGCCGgCGAACCUGaaacgCGuCACCGAACa- -3' miRNA: 3'- -UGGCaGUUUGGGCa----GU-GUGGCUUGgc -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 20101 | 0.66 | 0.800467 |
Target: 5'- gGuuGUCGGACCCGUCGgauacguacgaguuCACCacGCCu -3' miRNA: 3'- -UggCAGUUUGGGCAGU--------------GUGGcuUGGc -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 47154 | 0.67 | 0.793738 |
Target: 5'- gACCGUaCGAcucACCCcccaucgucGUCgACACgGAACCGg -3' miRNA: 3'- -UGGCA-GUU---UGGG---------CAG-UGUGgCUUGGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 50165 | 0.67 | 0.793738 |
Target: 5'- --gGUCG---CgGUCGCACCGGGCCGg -3' miRNA: 3'- uggCAGUuugGgCAGUGUGGCUUGGC- -5' |
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18048 | 3' | -53.9 | NC_004680.1 | + | 55969 | 0.67 | 0.783985 |
Target: 5'- cGCCGaucCAAcgcuGCCCaGcCGCACCGaAACCGg -3' miRNA: 3'- -UGGCa--GUU----UGGG-CaGUGUGGC-UUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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