Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18539 | 3' | -51.6 | NC_004682.1 | + | 50794 | 0.66 | 0.909484 |
Target: 5'- gGGUgUCGGGGGuauGCCagg--UACCCCa -3' miRNA: 3'- -CCAaAGCCCUUu--UGGgcguaGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 50595 | 1.14 | 0.001219 |
Target: 5'- gGGUUUCGGGAAAACCCGCAUCACCCCc -3' miRNA: 3'- -CCAAAGCCCUUUUGGGCGUAGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 50524 | 0.66 | 0.880763 |
Target: 5'- -----aGGGngAGGCCC-CAucucUCACCCCu -3' miRNA: 3'- ccaaagCCCu-UUUGGGcGU----AGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 48637 | 0.7 | 0.680934 |
Target: 5'- cGGUgucacccUUCGGGccuugugggccuugGGAACCCGUGUCuCCCUu -3' miRNA: 3'- -CCA-------AAGCCC--------------UUUUGGGCGUAGuGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 48484 | 0.7 | 0.685371 |
Target: 5'- cGGUgucuccggUGGGAccgauuGGGCCgGUAUCGCCCUu -3' miRNA: 3'- -CCAaa------GCCCU------UUUGGgCGUAGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 48368 | 0.67 | 0.838973 |
Target: 5'- cGGUgccUUCGaucuGGuc-GCCCaggaGCAUCGCCCCc -3' miRNA: 3'- -CCA---AAGC----CCuuuUGGG----CGUAGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 48104 | 0.7 | 0.704127 |
Target: 5'- --gUUCGGGuugacguuGCCguaucgaaucgccaUGCGUCACCCCa -3' miRNA: 3'- ccaAAGCCCuuu-----UGG--------------GCGUAGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 47591 | 0.66 | 0.888354 |
Target: 5'- cGGg--UGGGuu-GCCUGCGccgccgaugUCGCCCUg -3' miRNA: 3'- -CCaaaGCCCuuuUGGGCGU---------AGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 46377 | 0.72 | 0.584832 |
Target: 5'- ---cUCGcGGAGAGCCagaGCAgcgcgcuccuugUCGCCCCg -3' miRNA: 3'- ccaaAGC-CCUUUUGGg--CGU------------AGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 46328 | 0.66 | 0.880763 |
Target: 5'- cGGUcguaGGGAAGuACCuucuccgucauCGCGUCagGCCCCg -3' miRNA: 3'- -CCAaag-CCCUUU-UGG-----------GCGUAG--UGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 46196 | 0.66 | 0.895674 |
Target: 5'- ---cUCGGcGAGGucACCgGCcagguagccGUCGCCCCa -3' miRNA: 3'- ccaaAGCC-CUUU--UGGgCG---------UAGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 45700 | 0.66 | 0.909483 |
Target: 5'- uGGccUCGGaGAu-GCCCGCAgcaCGCgCCa -3' miRNA: 3'- -CCaaAGCC-CUuuUGGGCGUa--GUGgGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 44104 | 0.71 | 0.663115 |
Target: 5'- ---gUCGGGAGGAUCgGCccgGUCACCUUg -3' miRNA: 3'- ccaaAGCCCUUUUGGgCG---UAGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 42314 | 0.66 | 0.888354 |
Target: 5'- gGGUUgccgUCGuGGAAGACCUcuuGCucgAUCACCgCg -3' miRNA: 3'- -CCAA----AGC-CCUUUUGGG---CG---UAGUGGgG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 41629 | 0.69 | 0.729156 |
Target: 5'- ----gCGcGGAugGCUCGCAUCACCUg -3' miRNA: 3'- ccaaaGC-CCUuuUGGGCGUAGUGGGg -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 41300 | 0.71 | 0.618328 |
Target: 5'- cGGUcUCGGGGucgacGCCCaGCuccUCGCCCUu -3' miRNA: 3'- -CCAaAGCCCUuu---UGGG-CGu--AGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 40444 | 0.66 | 0.908153 |
Target: 5'- ----aCGGGAcgccgguaccuACCUGCGagACCCCg -3' miRNA: 3'- ccaaaGCCCUuu---------UGGGCGUagUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 39715 | 0.67 | 0.873704 |
Target: 5'- aGGUaccgcugCGGGuAGAACCCGagcagugcgaagacgAUCACCUCa -3' miRNA: 3'- -CCAaa-----GCCC-UUUUGGGCg--------------UAGUGGGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 38814 | 0.69 | 0.760981 |
Target: 5'- cGGgacugUCGGGAAGgcugcGCCgGUAUCGuuCCg -3' miRNA: 3'- -CCaa---AGCCCUUU-----UGGgCGUAGUggGG- -5' |
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18539 | 3' | -51.6 | NC_004682.1 | + | 35644 | 0.73 | 0.519162 |
Target: 5'- gGGggagGGGAAGGCCCGCcaaggaaACCCCu -3' miRNA: 3'- -CCaaagCCCUUUUGGGCGuag----UGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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