Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18552 | 5' | -60.8 | NC_004682.1 | + | 15430 | 0.74 | 0.129024 |
Target: 5'- aUCGCGaaGGACGaCGAGGGCaagcaGCGCGa -3' miRNA: 3'- gAGCGCggCUUGC-GCUCCUGg----CGCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 41860 | 0.74 | 0.126958 |
Target: 5'- --aGUGCCGGggccagcacagaccaGCGCGAGcuGGCCGCGCAg -3' miRNA: 3'- gagCGCGGCU---------------UGCGCUC--CUGGCGCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 41791 | 0.66 | 0.433157 |
Target: 5'- gUCaGCGCUGAaguacaGCGUcGGGACuCGCGCc -3' miRNA: 3'- gAG-CGCGGCU------UGCGcUCCUG-GCGCGu -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 19971 | 0.66 | 0.423934 |
Target: 5'- aUCGC-CCGcAACGCGAcuGCCGCGaCGg -3' miRNA: 3'- gAGCGcGGC-UUGCGCUccUGGCGC-GU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 46354 | 0.66 | 0.396988 |
Target: 5'- -gCcCGCCGggUGCGAGGgaacGCCuCGCGg -3' miRNA: 3'- gaGcGCGGCuuGCGCUCC----UGGcGCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 36238 | 0.67 | 0.388254 |
Target: 5'- gUCGCGguaCUGAGCGgGcAGGACCGgaGCGu -3' miRNA: 3'- gAGCGC---GGCUUGCgC-UCCUGGCg-CGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 3180 | 0.67 | 0.379646 |
Target: 5'- -gCGgGCCaGGGCGCGGGcaACCGCGUc -3' miRNA: 3'- gaGCgCGG-CUUGCGCUCc-UGGCGCGu -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 29903 | 0.67 | 0.371168 |
Target: 5'- -cCuCGCCGAACGCGccGAuCCgGCGCAg -3' miRNA: 3'- gaGcGCGGCUUGCGCucCU-GG-CGCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 8668 | 0.67 | 0.353788 |
Target: 5'- cCUCGCGUUcGGCGCGAgacaGGACUuccugaugaguaaGCGCAa -3' miRNA: 3'- -GAGCGCGGcUUGCGCU----CCUGG-------------CGCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 18116 | 0.68 | 0.330747 |
Target: 5'- uUCGCGCaGGGCGCGucGGACauCGCGg -3' miRNA: 3'- gAGCGCGgCUUGCGCu-CCUGgcGCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 36497 | 0.69 | 0.293662 |
Target: 5'- cCUCGCagcgccuccagaGCCGcACGgagguCGAGGACCGcCGCGu -3' miRNA: 3'- -GAGCG------------CGGCuUGC-----GCUCCUGGC-GCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 37205 | 0.69 | 0.293662 |
Target: 5'- uCUU-CGCCGcGC-CGAGGGCCGUGCu -3' miRNA: 3'- -GAGcGCGGCuUGcGCUCCUGGCGCGu -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 20003 | 0.69 | 0.273011 |
Target: 5'- gUCGCGCCuGGAgGCGcucccgagcaucGGGGCCG-GCAa -3' miRNA: 3'- gAGCGCGG-CUUgCGC------------UCCUGGCgCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 42138 | 0.69 | 0.259901 |
Target: 5'- cCUCGCGauGAGCGCGucguGGAUCGCuCGg -3' miRNA: 3'- -GAGCGCggCUUGCGCu---CCUGGCGcGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 9676 | 0.71 | 0.218037 |
Target: 5'- -aCGCGCUGGAuCGCaAGGcuGCCGCGUAc -3' miRNA: 3'- gaGCGCGGCUU-GCGcUCC--UGGCGCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 48722 | 0.71 | 0.207182 |
Target: 5'- -cCGCGCUGAaguagaGCGCGAGGuucgacagcuuCUGCGCGu -3' miRNA: 3'- gaGCGCGGCU------UGCGCUCCu----------GGCGCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 40345 | 0.72 | 0.177387 |
Target: 5'- -cCGCGUCGAGCGCGGucACCGCGa- -3' miRNA: 3'- gaGCGCGGCUUGCGCUccUGGCGCgu -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 29329 | 0.68 | 0.323062 |
Target: 5'- gUCgGUGCCG-ACGU--GGACCGCGUAg -3' miRNA: 3'- gAG-CGCGGCuUGCGcuCCUGGCGCGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 49944 | 0.67 | 0.362819 |
Target: 5'- gUUGCGUCGGACGCGuugauccGACCGUuuacGCAc -3' miRNA: 3'- gAGCGCGGCUUGCGCuc-----CUGGCG----CGU- -5' |
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18552 | 5' | -60.8 | NC_004682.1 | + | 20670 | 0.68 | 0.323062 |
Target: 5'- cCUgGCGUCGGuGCcCGAGGACaagGCGCAg -3' miRNA: 3'- -GAgCGCGGCU-UGcGCUCCUGg--CGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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