Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19554 | 5' | -53.4 | NC_004686.1 | + | 15055 | 0.66 | 0.795464 |
Target: 5'- -cGCaCGCUCAAUGCUuuUGACGcUCCa- -3' miRNA: 3'- uuCG-GUGAGUUGCGG--ACUGUuAGGcg -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 9548 | 0.66 | 0.795464 |
Target: 5'- aGGGCaCGUUCAuACGCCUGAgCGgcGUCUGCa -3' miRNA: 3'- -UUCG-GUGAGU-UGCGGACU-GU--UAGGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 23293 | 0.66 | 0.795464 |
Target: 5'- -uGCCGCaccUCccGCGCCUGACGAcaggacaagcaaUCCGa -3' miRNA: 3'- uuCGGUG---AGu-UGCGGACUGUU------------AGGCg -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 12128 | 0.66 | 0.782376 |
Target: 5'- -uGCCGCaacggcgggcaguuUCGGCGCC-GGCGGUcaggCCGCg -3' miRNA: 3'- uuCGGUG--------------AGUUGCGGaCUGUUA----GGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 42600 | 0.66 | 0.775212 |
Target: 5'- gGGGCUugUCuuCGCCgggGAUAAUgCCaGCg -3' miRNA: 3'- -UUCGGugAGuuGCGGa--CUGUUA-GG-CG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 37504 | 0.66 | 0.775212 |
Target: 5'- cGGCCACgugUAGCGCgCUucGGCGAUaCGCa -3' miRNA: 3'- uUCGGUGa--GUUGCG-GA--CUGUUAgGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 20254 | 0.67 | 0.754338 |
Target: 5'- -cGCUGCUCGuugaaaACGCCUGA----CCGCa -3' miRNA: 3'- uuCGGUGAGU------UGCGGACUguuaGGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 1718 | 0.67 | 0.743698 |
Target: 5'- -uGCUugUUGGCGCgaggGAgAGUCCGCg -3' miRNA: 3'- uuCGGugAGUUGCGga--CUgUUAGGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 33650 | 0.67 | 0.743698 |
Target: 5'- cGAGCCGUUUGACaccuCCUGGCcuUCCGCu -3' miRNA: 3'- -UUCGGUGAGUUGc---GGACUGuuAGGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 24336 | 0.67 | 0.73294 |
Target: 5'- -cGCCACcggCAGCGgCgGACAAggcgCCGUg -3' miRNA: 3'- uuCGGUGa--GUUGCgGaCUGUUa---GGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 3540 | 0.67 | 0.722075 |
Target: 5'- -cGCCGCguUCAGgGCCgcGGCAcgCUGCa -3' miRNA: 3'- uuCGGUG--AGUUgCGGa-CUGUuaGGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 38820 | 0.67 | 0.710017 |
Target: 5'- cGGCCACUacgucaaCGACGCCgcccGCAagAUCUGCu -3' miRNA: 3'- uUCGGUGA-------GUUGCGGac--UGU--UAGGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 33158 | 0.67 | 0.700077 |
Target: 5'- uAGCCAC-CAACGCCacACAAaCCGg -3' miRNA: 3'- uUCGGUGaGUUGCGGacUGUUaGGCg -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 17223 | 0.67 | 0.700077 |
Target: 5'- -cGUUAUUCGGaucucUGCCUGAgGAUCUGCg -3' miRNA: 3'- uuCGGUGAGUU-----GCGGACUgUUAGGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 21238 | 0.67 | 0.700077 |
Target: 5'- -cGCgGCgcggaaCAACGCCUGACcacccuuagCCGCg -3' miRNA: 3'- uuCGgUGa-----GUUGCGGACUGuua------GGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 38405 | 0.68 | 0.688968 |
Target: 5'- -cGCUGCUCAuCGCCgcgGGCAucAUCgGCc -3' miRNA: 3'- uuCGGUGAGUuGCGGa--CUGU--UAGgCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 19950 | 0.68 | 0.688968 |
Target: 5'- cGGCCAUgcccugagCGACGaUCUGGuCGAUCUGCg -3' miRNA: 3'- uUCGGUGa-------GUUGC-GGACU-GUUAGGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 39662 | 0.68 | 0.655343 |
Target: 5'- --uCCACgaCAACGCC--ACGAUCCGCu -3' miRNA: 3'- uucGGUGa-GUUGCGGacUGUUAGGCG- -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 34073 | 0.68 | 0.644075 |
Target: 5'- aAGGCCGC-CAACGgCUGGucCGAUCCa- -3' miRNA: 3'- -UUCGGUGaGUUGCgGACU--GUUAGGcg -5' |
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19554 | 5' | -53.4 | NC_004686.1 | + | 44008 | 0.69 | 0.610243 |
Target: 5'- cAAGCCACUCggUGaUCUcuaGACAGucUCCGUg -3' miRNA: 3'- -UUCGGUGAGuuGC-GGA---CUGUU--AGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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