Results 1 - 20 of 74 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20412 | 5' | -52.3 | NC_004688.1 | + | 65210 | 0.65 | 0.971816 |
Target: 5'- aGGCCCAggAAAUGCuucuuguagcCGUCAUUGACc -3' miRNA: 3'- -CCGGGUa-UUUGCGcua-------GCGGUAGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 21145 | 0.66 | 0.969413 |
Target: 5'- aGGCCgAUAAcCGCGGagGCaGUCGGg -3' miRNA: 3'- -CCGGgUAUUuGCGCUagCGgUAGCUg -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 78763 | 0.66 | 0.969413 |
Target: 5'- uGCCCAUAAACacucCGAgucggUGCCccagAUCGGCu -3' miRNA: 3'- cCGGGUAUUUGc---GCUa----GCGG----UAGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 102827 | 0.66 | 0.969413 |
Target: 5'- uGGCgaUCGUGAGCGCuAUUGCCGaagaaUUGGCg -3' miRNA: 3'- -CCG--GGUAUUUGCGcUAGCGGU-----AGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 26028 | 0.66 | 0.969102 |
Target: 5'- aGGCCCGUGGGCauaccggugcugaGCGGUCcgccuGCCAUacccccgGGCa -3' miRNA: 3'- -CCGGGUAUUUG-------------CGCUAG-----CGGUAg------CUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 55999 | 0.66 | 0.966207 |
Target: 5'- cGGUUCAcGAugGCGAUCgGCUuUCGuACa -3' miRNA: 3'- -CCGGGUaUUugCGCUAG-CGGuAGC-UG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 32152 | 0.66 | 0.966207 |
Target: 5'- uGCCCcgAccgcAGCGCcuucaacgUGCCAUCGGCa -3' miRNA: 3'- cCGGGuaU----UUGCGcua-----GCGGUAGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 11397 | 0.66 | 0.966207 |
Target: 5'- cGGUgCCGUcAGCGCGGU-GCCAcCGAUc -3' miRNA: 3'- -CCG-GGUAuUUGCGCUAgCGGUaGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 41924 | 0.66 | 0.966207 |
Target: 5'- uGCCCGgccaGAACG-GAUCGCgguaGUUGGCg -3' miRNA: 3'- cCGGGUa---UUUGCgCUAGCGg---UAGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 21087 | 0.66 | 0.966207 |
Target: 5'- uGGCCUu--GACGCGGaacuuccagcUCGCCGaagaggUGACg -3' miRNA: 3'- -CCGGGuauUUGCGCU----------AGCGGUa-----GCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 105509 | 0.66 | 0.962772 |
Target: 5'- cGGCCCgAUGAAcCGCaaGcgCGCCAUgaaguuugcCGGCg -3' miRNA: 3'- -CCGGG-UAUUU-GCG--CuaGCGGUA---------GCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 35552 | 0.66 | 0.959101 |
Target: 5'- -cUCCAUu--CGCcgGAUCGCCAUCGuGCg -3' miRNA: 3'- ccGGGUAuuuGCG--CUAGCGGUAGC-UG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 91170 | 0.66 | 0.959101 |
Target: 5'- aGCCCuc-GAUGauGUCGCCAacaUCGGCa -3' miRNA: 3'- cCGGGuauUUGCgcUAGCGGU---AGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 22898 | 0.66 | 0.959101 |
Target: 5'- uGCCgAU-GACGCccaGGUCGCCGccgCGGCg -3' miRNA: 3'- cCGGgUAuUUGCG---CUAGCGGUa--GCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 82572 | 0.66 | 0.955189 |
Target: 5'- cGGCgUCGUAGAUGCGcgcCGCCAgcUgGACg -3' miRNA: 3'- -CCG-GGUAUUUGCGCua-GCGGU--AgCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 54324 | 0.66 | 0.955189 |
Target: 5'- aGGCCCA-----GCGGuacUCGCCGUUGGu -3' miRNA: 3'- -CCGGGUauuugCGCU---AGCGGUAGCUg -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 60627 | 0.66 | 0.955189 |
Target: 5'- uGCCagu-AGCgGCuGUCGCUAUCGACg -3' miRNA: 3'- cCGGguauUUG-CGcUAGCGGUAGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 10452 | 0.66 | 0.955189 |
Target: 5'- cGGUCCAggu-CGCGGuauuccUCGCCGaccUUGGCa -3' miRNA: 3'- -CCGGGUauuuGCGCU------AGCGGU---AGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 65449 | 0.67 | 0.951031 |
Target: 5'- uGGCCCuccgGGAUGCGGaCGuauCCGUUGAUa -3' miRNA: 3'- -CCGGGua--UUUGCGCUaGC---GGUAGCUG- -5' |
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20412 | 5' | -52.3 | NC_004688.1 | + | 5738 | 0.67 | 0.951031 |
Target: 5'- gGGCCCGgguGACGCGGcCGaggggCGGCa -3' miRNA: 3'- -CCGGGUau-UUGCGCUaGCggua-GCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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