Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20421 | 3' | -54 | NC_004688.1 | + | 20485 | 0.68 | 0.829091 |
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Target: 5'- aGCGCAcg-GCGCGCUUG-CUCuGGGCg -3' miRNA: 3'- gCGCGUaugCGUGCGAGCuGAG-CUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 19222 | 0.66 | 0.92308 |
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Target: 5'- gGCGaguucgGCgGC-CGUUCGugUCGAACg -3' miRNA: 3'- gCGCgua---UG-CGuGCGAGCugAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 11273 | 1.11 | 0.002549 |
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Target: 5'- gCGCGCAUACGCACGCUCGACUCGAACa -3' miRNA: 3'- -GCGCGUAUGCGUGCGAGCUGAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 10541 | 0.68 | 0.837655 |
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Target: 5'- -uCGCGUAgGUGCGCcCGGCgCGAGCg -3' miRNA: 3'- gcGCGUAUgCGUGCGaGCUGaGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 9254 | 0.66 | 0.928612 |
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Target: 5'- uCGCGCucuucCGCgGCGCUUucCUCGGGCu -3' miRNA: 3'- -GCGCGuau--GCG-UGCGAGcuGAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 5939 | 0.66 | 0.911255 |
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Target: 5'- cCGCGcCGUGCGCcUGCUUGGcCUUGuACu -3' miRNA: 3'- -GCGC-GUAUGCGuGCGAGCU-GAGCuUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 5344 | 0.66 | 0.93593 |
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Target: 5'- cCGCGgGUccACGCuguCGCUguacucguauccgagCGACUCGAGg -3' miRNA: 3'- -GCGCgUA--UGCGu--GCGA---------------GCUGAGCUUg -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 4068 | 0.73 | 0.567441 |
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Target: 5'- cCGCgGCGUAgGCGCGCaUCGuCUCGAu- -3' miRNA: 3'- -GCG-CGUAUgCGUGCG-AGCuGAGCUug -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 451 | 0.68 | 0.854179 |
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Target: 5'- uCGCGCcUGCGguaGCGCUCucGCUCGcGCg -3' miRNA: 3'- -GCGCGuAUGCg--UGCGAGc-UGAGCuUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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