Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21040 | 3' | -52.6 | NC_004745.1 | + | 5423 | 0.7 | 0.468199 |
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Target: 5'- gCGUCUGGauACCGGCGAUAUugCCGGUg -3' miRNA: 3'- aGUGGACUauUGGCCGCUAUG--GGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 5947 | 0.7 | 0.457606 |
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Target: 5'- aUCACCUGAcacUGcACCGGCGuccaccGCCCGa- -3' miRNA: 3'- -AGUGGACU---AU-UGGCCGCua----UGGGUcg -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 5976 | 0.7 | 0.447142 |
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Target: 5'- uUCACCcucacUGACCGGCGugccGCCCuGGCg -3' miRNA: 3'- -AGUGGacu--AUUGGCCGCua--UGGG-UCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 9356 | 0.7 | 0.447142 |
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Target: 5'- aUCAUCUGuacauccucACCGGUGAaGCCgAGCa -3' miRNA: 3'- -AGUGGACuau------UGGCCGCUaUGGgUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 3524 | 0.7 | 0.446103 |
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Target: 5'- gUUAUCUGGUGggGCUGGCcgucaccGAUgACCCGGCa -3' miRNA: 3'- -AGUGGACUAU--UGGCCG-------CUA-UGGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 10124 | 0.71 | 0.406672 |
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Target: 5'- -aGCCaGAguAUCGGCGAUAUCCuGCg -3' miRNA: 3'- agUGGaCUauUGGCCGCUAUGGGuCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 8235 | 0.71 | 0.387317 |
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Target: 5'- -uGCCUGAUGcugugcGCCGGU--UGCaCCAGCg -3' miRNA: 3'- agUGGACUAU------UGGCCGcuAUG-GGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 7462 | 0.77 | 0.177324 |
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Target: 5'- cUCACgCUGAccgUAACCGGC---ACCCGGCa -3' miRNA: 3'- -AGUG-GACU---AUUGGCCGcuaUGGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 6976 | 1.14 | 0.000414 |
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Target: 5'- aUCACCUGAUAACCGGCGAUACCCAGCa -3' miRNA: 3'- -AGUGGACUAUUGGCCGCUAUGGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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