Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21040 | 3' | -52.6 | NC_004745.1 | + | 14249 | 0.68 | 0.568385 |
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Target: 5'- cCGCCaaGAUcACUGGauguucCGAUGCCCAGUg -3' miRNA: 3'- aGUGGa-CUAuUGGCC------GCUAUGGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 1749 | 0.69 | 0.545498 |
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Target: 5'- -uGCgUGGUAcuggcaccGCCGGUGGUGgCCGGUg -3' miRNA: 3'- agUGgACUAU--------UGGCCGCUAUgGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 21057 | 0.69 | 0.534165 |
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Target: 5'- gCAuCCUG-UAACUGGCGGUcaagcugugucCCCGGCg -3' miRNA: 3'- aGU-GGACuAUUGGCCGCUAu----------GGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 5574 | 0.69 | 0.51956 |
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Target: 5'- cCACCUccucGGUGAacauguaCGGCGGggugcggcgguguuUACCCGGCa -3' miRNA: 3'- aGUGGA----CUAUUg------GCCGCU--------------AUGGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 5976 | 0.7 | 0.447142 |
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Target: 5'- uUCACCcucacUGACCGGCGugccGCCCuGGCg -3' miRNA: 3'- -AGUGGacu--AUUGGCCGCua--UGGG-UCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 9356 | 0.7 | 0.447142 |
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Target: 5'- aUCAUCUGuacauccucACCGGUGAaGCCgAGCa -3' miRNA: 3'- -AGUGGACuau------UGGCCGCUaUGGgUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 7462 | 0.77 | 0.177324 |
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Target: 5'- cUCACgCUGAccgUAACCGGC---ACCCGGCa -3' miRNA: 3'- -AGUG-GACU---AUUGGCCGcuaUGGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 6976 | 1.14 | 0.000414 |
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Target: 5'- aUCACCUGAUAACCGGCGAUACCCAGCa -3' miRNA: 3'- -AGUGGACUAUUGGCCGCUAUGGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 10203 | 0.66 | 0.718958 |
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Target: 5'- aUCACCcgUGAgccGACCGGUG-UGCgCAGg -3' miRNA: 3'- -AGUGG--ACUa--UUGGCCGCuAUGgGUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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