Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21040 | 3' | -52.6 | NC_004745.1 | + | 9671 | 0.7 | 0.478917 |
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Target: 5'- -uGCCgggGAUuuauuCCGGCGAUaACCCcGCg -3' miRNA: 3'- agUGGa--CUAuu---GGCCGCUA-UGGGuCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 10124 | 0.71 | 0.406672 |
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Target: 5'- -aGCCaGAguAUCGGCGAUAUCCuGCg -3' miRNA: 3'- agUGGaCUauUGGCCGCUAUGGGuCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 10203 | 0.66 | 0.718958 |
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Target: 5'- aUCACCcgUGAgccGACCGGUG-UGCgCAGg -3' miRNA: 3'- -AGUGG--ACUa--UUGGCCGCuAUGgGUCg -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 14249 | 0.68 | 0.568385 |
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Target: 5'- cCGCCaaGAUcACUGGauguucCGAUGCCCAGUg -3' miRNA: 3'- aGUGGa-CUAuUGGCC------GCUAUGGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 19899 | 0.66 | 0.673052 |
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Target: 5'- -gGCCaguuuUGAgaaaAACCGGCucGGUacuGCCCAGCa -3' miRNA: 3'- agUGG-----ACUa---UUGGCCG--CUA---UGGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 20398 | 0.68 | 0.603127 |
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Target: 5'- gCGCCUgcugccgcGAUAauggcauugauGCCGGUGAUAaCCGGCc -3' miRNA: 3'- aGUGGA--------CUAU-----------UGGCCGCUAUgGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 21057 | 0.69 | 0.534165 |
|
Target: 5'- gCAuCCUG-UAACUGGCGGUcaagcugugucCCCGGCg -3' miRNA: 3'- aGU-GGACuAUUGGCCGCUAu----------GGGUCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 22640 | 0.66 | 0.684626 |
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Target: 5'- -aGCCUGAcacucUGACCGGUGuuuCCguGUa -3' miRNA: 3'- agUGGACU-----AUUGGCCGCuauGGguCG- -5' |
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| 21040 | 3' | -52.6 | NC_004745.1 | + | 27333 | 0.66 | 0.673052 |
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Target: 5'- aUCACCUGuAUGgucauguuGCUGGCGcUGCaCgAGCu -3' miRNA: 3'- -AGUGGAC-UAU--------UGGCCGCuAUG-GgUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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