Results 1 - 20 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21196 | 5' | -57.8 | NC_004778.3 | + | 7828 | 0.66 | 0.802853 |
Target: 5'- -----gACGGCGUggaCCGcGCGCUGCAg -3' miRNA: 3'- uuuuagUGCCGCGag-GGC-UGCGGCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 7726 | 0.66 | 0.801056 |
Target: 5'- ----cUACGGCGCgccgacaacgugCCCGACGCgggccUGCAc -3' miRNA: 3'- uuuuaGUGCCGCGa-----------GGGCUGCG-----GCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 43846 | 0.66 | 0.793805 |
Target: 5'- ----gCGCGGC-CgUCCGACGCgGCAa -3' miRNA: 3'- uuuuaGUGCCGcGaGGGCUGCGgCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 25784 | 0.66 | 0.784608 |
Target: 5'- gGAGAaCACGGUGCUacaCCgCGACGgCGUg -3' miRNA: 3'- -UUUUaGUGCCGCGA---GG-GCUGCgGCGu -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 85692 | 0.66 | 0.781822 |
Target: 5'- -cGAUCGCGcGCgGUggacacaugaugugUCCCGGCGCgGCGc -3' miRNA: 3'- uuUUAGUGC-CG-CG--------------AGGGCUGCGgCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 21013 | 0.66 | 0.775273 |
Target: 5'- ----cCGCGGCGCaccagcgcgUCCaccuGCGCCGCGg -3' miRNA: 3'- uuuuaGUGCCGCG---------AGGgc--UGCGGCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 9448 | 0.66 | 0.775273 |
Target: 5'- ----aCACGGcCGUcgUCUCGGCGgCGCAg -3' miRNA: 3'- uuuuaGUGCC-GCG--AGGGCUGCgGCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 52925 | 0.66 | 0.765808 |
Target: 5'- --cGUCGcCGGCGCUCugCCGuuucaGCCGCc -3' miRNA: 3'- uuuUAGU-GCCGCGAG--GGCug---CGGCGu -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 85119 | 0.66 | 0.756222 |
Target: 5'- ---cUCGCGGCGCcgCUCGuCGCUgGCGg -3' miRNA: 3'- uuuuAGUGCCGCGa-GGGCuGCGG-CGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 61068 | 0.66 | 0.756222 |
Target: 5'- cGAAUCGCGGCGCcgaCUGAaUGCgCGCu -3' miRNA: 3'- uUUUAGUGCCGCGag-GGCU-GCG-GCGu -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 85271 | 0.66 | 0.756222 |
Target: 5'- ----aCACGGCGCccgcguccaUgCCGuCGCCGCc -3' miRNA: 3'- uuuuaGUGCCGCG---------AgGGCuGCGGCGu -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 32908 | 0.67 | 0.746525 |
Target: 5'- -uGGUCACuuggcaGGCGCUCCCauuuuCGcCCGCGu -3' miRNA: 3'- uuUUAGUG------CCGCGAGGGcu---GC-GGCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 33683 | 0.67 | 0.736726 |
Target: 5'- ----gCGCGGauaGCgCCCGGCGCgGCu -3' miRNA: 3'- uuuuaGUGCCg--CGaGGGCUGCGgCGu -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 59227 | 0.67 | 0.736726 |
Target: 5'- --uGUC-CGGCGCU-UgGGCGCCGUAg -3' miRNA: 3'- uuuUAGuGCCGCGAgGgCUGCGGCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 34196 | 0.67 | 0.736726 |
Target: 5'- ----cCGCGGCGCaccaugCCCGAgucCGCgGCGa -3' miRNA: 3'- uuuuaGUGCCGCGa-----GGGCU---GCGgCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 119153 | 0.67 | 0.736726 |
Target: 5'- cGAAGUCGCGaGCGCcUuuGcaauGCGCCGCc -3' miRNA: 3'- -UUUUAGUGC-CGCGaGggC----UGCGGCGu -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 91812 | 0.67 | 0.736726 |
Target: 5'- -cGGUCACGGCGC-CgCCGAuuuuuuuacCGCUGUg -3' miRNA: 3'- uuUUAGUGCCGCGaG-GGCU---------GCGGCGu -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 15546 | 0.67 | 0.726836 |
Target: 5'- ----cCGCGGCGCcgccuucgCCCGACG-CGCc -3' miRNA: 3'- uuuuaGUGCCGCGa-------GGGCUGCgGCGu -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 94981 | 0.67 | 0.716861 |
Target: 5'- ----cCGCGguGCGCUCCuCGACG-CGCAg -3' miRNA: 3'- uuuuaGUGC--CGCGAGG-GCUGCgGCGU- -5' |
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21196 | 5' | -57.8 | NC_004778.3 | + | 43132 | 0.67 | 0.716861 |
Target: 5'- ---uUCgACGGCGC-CgCGgcGCGCCGCAc -3' miRNA: 3'- uuuuAG-UGCCGCGaGgGC--UGCGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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