Results 1 - 20 of 72 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21205 | 3' | -65.5 | NC_004778.3 | + | 95005 | 0.66 | 0.486545 |
Target: 5'- gCguGCCCGuguacagcaguacggGCACGccGCGCUGCCGGagCAg -3' miRNA: 3'- -GguCGGGC---------------UGUGC--CGCGGCGGCCg-GU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 117444 | 0.66 | 0.474048 |
Target: 5'- -aAGCggCGGCGCGGCaGCgGCCuGCCAc -3' miRNA: 3'- ggUCGg-GCUGUGCCG-CGgCGGcCGGU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 96600 | 0.66 | 0.474048 |
Target: 5'- gCUAGCCa-GCGCGGCgGCCGCUaGCgCGa -3' miRNA: 3'- -GGUCGGgcUGUGCCG-CGGCGGcCG-GU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 82668 | 0.66 | 0.474048 |
Target: 5'- --cGCuuGGCGCgGGCGCCaaGCC-GCCAa -3' miRNA: 3'- gguCGggCUGUG-CCGCGG--CGGcCGGU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 45590 | 0.66 | 0.46259 |
Target: 5'- uCCAgcGCCUGGCGCGccuucugcauagccGCGCCcucgucGCCGGCg- -3' miRNA: 3'- -GGU--CGGGCUGUGC--------------CGCGG------CGGCCGgu -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 15079 | 0.66 | 0.456481 |
Target: 5'- gCCGGCCuCGccCGCGGCGC-GCagGGUCAa -3' miRNA: 3'- -GGUCGG-GCu-GUGCCGCGgCGg-CCGGU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 47709 | 0.66 | 0.455612 |
Target: 5'- gCCGcGCCgGAUAUcgcaGGCGCCGCuacuaagCGGUCAu -3' miRNA: 3'- -GGU-CGGgCUGUG----CCGCGGCG-------GCCGGU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 33661 | 0.66 | 0.447829 |
Target: 5'- gCAGCgCGGC-CGGCGCgcUGCCGcGCgGa -3' miRNA: 3'- gGUCGgGCUGuGCCGCG--GCGGC-CGgU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 33069 | 0.66 | 0.447829 |
Target: 5'- -gAGCCUGGCgcaacggugcaGCGGCccGCUGCCGcGCUAc -3' miRNA: 3'- ggUCGGGCUG-----------UGCCG--CGGCGGC-CGGU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 85715 | 0.66 | 0.446969 |
Target: 5'- aUguGuCCCGGCGCGGCGCCcCCGuaauuaaGCUu -3' miRNA: 3'- -GguC-GGGCUGUGCCGCGGcGGC-------CGGu -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 68072 | 0.66 | 0.439269 |
Target: 5'- cUCAGCgaCGACGCgccGGCGCCGuCCGcacaugaaGCCGa -3' miRNA: 3'- -GGUCGg-GCUGUG---CCGCGGC-GGC--------CGGU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 32790 | 0.67 | 0.430804 |
Target: 5'- aCuGCgCGaACAUGGaGUCGCUGGCCAu -3' miRNA: 3'- gGuCGgGC-UGUGCCgCGGCGGCCGGU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 101644 | 0.67 | 0.430804 |
Target: 5'- gCGGCCCGugcAUGCGGUGCagCGCgGGCa- -3' miRNA: 3'- gGUCGGGC---UGUGCCGCG--GCGgCCGgu -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 10683 | 0.67 | 0.414163 |
Target: 5'- gCCaAGCCCGACGaugaaGGCGCCa-CGGUa- -3' miRNA: 3'- -GG-UCGGGCUGUg----CCGCGGcgGCCGgu -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 8005 | 0.67 | 0.414163 |
Target: 5'- gCGGCgCCGACgGCGGCGaca-CGGCCGa -3' miRNA: 3'- gGUCG-GGCUG-UGCCGCggcgGCCGGU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 100097 | 0.67 | 0.414163 |
Target: 5'- aCAGCCgcuUGGCGCaccccaGCGCgCGCCGcGCCAc -3' miRNA: 3'- gGUCGG---GCUGUGc-----CGCG-GCGGC-CGGU- -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 119165 | 0.67 | 0.414163 |
Target: 5'- --cGCCUuuGCAauGCGCCGCCGGUCc -3' miRNA: 3'- gguCGGGc-UGUgcCGCGGCGGCCGGu -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 33695 | 0.67 | 0.403562 |
Target: 5'- cCCGGCgCGGCuccaaaucuugaugGCGGgGCCGUcgCGGCUg -3' miRNA: 3'- -GGUCGgGCUG--------------UGCCgCGGCG--GCCGGu -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 108426 | 0.67 | 0.397925 |
Target: 5'- gCAGgUgGACGCGcugguGCGCCGCgGGCUg -3' miRNA: 3'- gGUCgGgCUGUGC-----CGCGGCGgCCGGu -5' |
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21205 | 3' | -65.5 | NC_004778.3 | + | 110134 | 0.67 | 0.38996 |
Target: 5'- gCCGGUCCGuAUACGGCacagugcaGCUGCCGuaCGa -3' miRNA: 3'- -GGUCGGGC-UGUGCCG--------CGGCGGCcgGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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