Results 1 - 20 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21212 | 3' | -55.7 | NC_004778.3 | + | 21130 | 0.66 | 0.890404 |
Target: 5'- uCGCCgACGUgcCGcuCUGCGGCGUCGGGa -3' miRNA: 3'- uGCGG-UGCAa-GCu-GACGCUGCGGUUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 118106 | 0.66 | 0.890404 |
Target: 5'- uCGCCAUcgaggaGUuugacaaaaUCGGCgGCGGCGCCGGu -3' miRNA: 3'- uGCGGUG------CA---------AGCUGaCGCUGCGGUUu -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 85285 | 0.66 | 0.890404 |
Target: 5'- uCGCCcaaGUUCGGCcguuuuUGCucGGCGCCAAAg -3' miRNA: 3'- uGCGGug-CAAGCUG------ACG--CUGCGGUUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 49399 | 0.66 | 0.88833 |
Target: 5'- aGCGCCGCGUacUCGguauACUGCuGAaacauguauugcgcCGCCGAc -3' miRNA: 3'- -UGCGGUGCA--AGC----UGACG-CU--------------GCGGUUu -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 27678 | 0.66 | 0.883409 |
Target: 5'- uACGCUuCGggcaCGACgaGCGugGCCAGu -3' miRNA: 3'- -UGCGGuGCaa--GCUGa-CGCugCGGUUu -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 38078 | 0.66 | 0.868727 |
Target: 5'- aAUGCCGCug-CGGC-GCGACGCgCAAc -3' miRNA: 3'- -UGCGGUGcaaGCUGaCGCUGCG-GUUu -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 44316 | 0.66 | 0.868727 |
Target: 5'- cAUGCC-CGccagCGACgaGCGGCGCCGc- -3' miRNA: 3'- -UGCGGuGCaa--GCUGa-CGCUGCGGUuu -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 119555 | 0.66 | 0.868727 |
Target: 5'- cGCGCC-CGggCGGCacuuugGCGGCGCguAAg -3' miRNA: 3'- -UGCGGuGCaaGCUGa-----CGCUGCGguUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 2511 | 0.66 | 0.861051 |
Target: 5'- aGCGCaGCGUggccgaagugcUCGACuuguccagcaUGCGGCGCCGc- -3' miRNA: 3'- -UGCGgUGCA-----------AGCUG----------ACGCUGCGGUuu -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 62022 | 0.66 | 0.861051 |
Target: 5'- gAUGUCuGCGgacgcCGAgaGCGGCGCCAAAa -3' miRNA: 3'- -UGCGG-UGCaa---GCUgaCGCUGCGGUUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 93176 | 0.66 | 0.85316 |
Target: 5'- gGC-CCGCGUugUUGACcGCGuugGCGCCAAAa -3' miRNA: 3'- -UGcGGUGCA--AGCUGaCGC---UGCGGUUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 100642 | 0.66 | 0.85316 |
Target: 5'- cCGCgGCGUUUccUUGCGAUGCUAAAa -3' miRNA: 3'- uGCGgUGCAAGcuGACGCUGCGGUUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 38296 | 0.66 | 0.85316 |
Target: 5'- uGCGCCAgCGcgcacgCGACcGCG-CGCCGAGc -3' miRNA: 3'- -UGCGGU-GCaa----GCUGaCGCuGCGGUUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 49329 | 0.66 | 0.85316 |
Target: 5'- gGCGCCGCacaaCGugUGCGGCaCCAc- -3' miRNA: 3'- -UGCGGUGcaa-GCugACGCUGcGGUuu -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 117076 | 0.67 | 0.836761 |
Target: 5'- cAUGUUuCGUUCGACUGUGACcaCCAGGu -3' miRNA: 3'- -UGCGGuGCAAGCUGACGCUGc-GGUUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 38102 | 0.67 | 0.828266 |
Target: 5'- aGCGUCuccacagacGCGUUgaCGugUGCGuGCGCCAAAu -3' miRNA: 3'- -UGCGG---------UGCAA--GCugACGC-UGCGGUUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 115163 | 0.67 | 0.819586 |
Target: 5'- -gGCCACGUU-GACUucGCGGC-CCAAAa -3' miRNA: 3'- ugCGGUGCAAgCUGA--CGCUGcGGUUU- -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 128451 | 0.67 | 0.819586 |
Target: 5'- cCGCCACGcgUUGgaggccGCUGCuGCGCCGAu -3' miRNA: 3'- uGCGGUGCa-AGC------UGACGcUGCGGUUu -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 83113 | 0.67 | 0.819586 |
Target: 5'- cGCGCCGCGaUCGACUGguuacccaaCGugGUgAGg -3' miRNA: 3'- -UGCGGUGCaAGCUGAC---------GCugCGgUUu -5' |
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21212 | 3' | -55.7 | NC_004778.3 | + | 113892 | 0.67 | 0.810729 |
Target: 5'- gGCGCguCGggCGAagGCGGCGCCGc- -3' miRNA: 3'- -UGCGguGCaaGCUgaCGCUGCGGUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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