Results 1 - 20 of 56 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21219 | 3' | -58.2 | NC_004778.3 | + | 30721 | 0.66 | 0.825251 |
Target: 5'- cACGcUGCGGcCGCgGCCGGCUgccaucgaaaguucgCGUCGc -3' miRNA: 3'- -UGCuGCGCCuGUGgUGGUCGA---------------GCAGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 121580 | 0.66 | 0.821891 |
Target: 5'- --aACGUGGGCGCCACCGcGCUUa--- -3' miRNA: 3'- ugcUGCGCCUGUGGUGGU-CGAGcagc -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 14508 | 0.66 | 0.821891 |
Target: 5'- -aGGcCGCGGuGCGCaCGCCGGCUUG-CGc -3' miRNA: 3'- ugCU-GCGCC-UGUG-GUGGUCGAGCaGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 113901 | 0.66 | 0.813376 |
Target: 5'- gGCGAagGCGG-CGCCGCgGGUUCcUCGc -3' miRNA: 3'- -UGCUg-CGCCuGUGGUGgUCGAGcAGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 1654 | 0.66 | 0.813376 |
Target: 5'- aGCGACGCGucguuGGCACCuuCGGUUUGggugCGg -3' miRNA: 3'- -UGCUGCGC-----CUGUGGugGUCGAGCa---GC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 10068 | 0.66 | 0.813376 |
Target: 5'- cCGACGCaGuaaACugCGCCAGCUCa--- -3' miRNA: 3'- uGCUGCGcC---UGugGUGGUCGAGcagc -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 52856 | 0.66 | 0.8047 |
Target: 5'- aGCGAaaaUGCGGAgGCCAUuCAGCUUGa-- -3' miRNA: 3'- -UGCU---GCGCCUgUGGUG-GUCGAGCagc -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 8483 | 0.66 | 0.8047 |
Target: 5'- -gGGCGCGGcccGCGCCccGCCAGUcaUCGUa- -3' miRNA: 3'- ugCUGCGCC---UGUGG--UGGUCG--AGCAgc -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 113285 | 0.66 | 0.8047 |
Target: 5'- cGCGACGCGcACGUCGCaCAGCgaGUCGa -3' miRNA: 3'- -UGCUGCGCcUGUGGUG-GUCGagCAGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 43856 | 0.66 | 0.795872 |
Target: 5'- cCGACGCGGcaACAgguGCCGGCUCGg-- -3' miRNA: 3'- uGCUGCGCC--UGUgg-UGGUCGAGCagc -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 88709 | 0.66 | 0.795872 |
Target: 5'- aGCGGCGCGuGCGCUcCCaauugAGCUUGUUGu -3' miRNA: 3'- -UGCUGCGCcUGUGGuGG-----UCGAGCAGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 103660 | 0.66 | 0.7869 |
Target: 5'- aGCGugGCGGcaACGCgGUCAGCUUaUCGa -3' miRNA: 3'- -UGCugCGCC--UGUGgUGGUCGAGcAGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 97165 | 0.66 | 0.7869 |
Target: 5'- cCGACaCGG-CGCCGCCGGCcagCGUgCGc -3' miRNA: 3'- uGCUGcGCCuGUGGUGGUCGa--GCA-GC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 82612 | 0.66 | 0.7869 |
Target: 5'- gAUGAgGCGGcgaGCCGCCGcGCgauagCGUCGc -3' miRNA: 3'- -UGCUgCGCCug-UGGUGGU-CGa----GCAGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 97402 | 0.67 | 0.777793 |
Target: 5'- gGCGGCGCgcaaGGAaGCCGCCGGUUCu--- -3' miRNA: 3'- -UGCUGCG----CCUgUGGUGGUCGAGcagc -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 91332 | 0.67 | 0.759206 |
Target: 5'- -gGACGCGGACggcguGCgCGCUAGCgugcuguugcgCGUCGc -3' miRNA: 3'- ugCUGCGCCUG-----UG-GUGGUCGa----------GCAGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 89888 | 0.67 | 0.759206 |
Target: 5'- aGCGugGUGGGCGCaAgCGGCUUG-CGc -3' miRNA: 3'- -UGCugCGCCUGUGgUgGUCGAGCaGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 16927 | 0.67 | 0.748793 |
Target: 5'- cGCGAgccguuaCGCGGcGCACaugCACCGGCUCGaguucUCGg -3' miRNA: 3'- -UGCU-------GCGCC-UGUG---GUGGUCGAGC-----AGC- -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 120697 | 0.67 | 0.740182 |
Target: 5'- gACGGgGUGGcGCGCCacggcGCCGGCggCGUCa -3' miRNA: 3'- -UGCUgCGCC-UGUGG-----UGGUCGa-GCAGc -5' |
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21219 | 3' | -58.2 | NC_004778.3 | + | 80806 | 0.67 | 0.740182 |
Target: 5'- -aGACGCGGcaACgGCCGGUcgCGUCGc -3' miRNA: 3'- ugCUGCGCCugUGgUGGUCGa-GCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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