Results 1 - 20 of 90 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21376 | 3' | -59 | NC_004812.1 | + | 148412 | 0.66 | 0.81053 |
Target: 5'- gGCCCccggccgCUCUCggCCGGACCCCggCC-Cg -3' miRNA: 3'- -CGGGaa-----GAGAG--GGUCUGGGGa-GGaGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 117511 | 0.66 | 0.81053 |
Target: 5'- gGCCCccggccgCUCUCggCCGGACCCCggCC-Cg -3' miRNA: 3'- -CGGGaa-----GAGAG--GGUCUGGGGa-GGaGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 151457 | 0.66 | 0.81053 |
Target: 5'- cGCCCcUCUCUgcuucuggCCCGcGGCCCUcccgCCUCc -3' miRNA: 3'- -CGGGaAGAGA--------GGGU-CUGGGGa---GGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 120556 | 0.66 | 0.81053 |
Target: 5'- cGCCCcUCUCUgcuucuggCCCGcGGCCCUcccgCCUCc -3' miRNA: 3'- -CGGGaAGAGA--------GGGU-CUGGGGa---GGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 128795 | 0.66 | 0.801871 |
Target: 5'- cGCCCgUCgaacccccgCUCCCcccccGGCCCCgcCCUCUc -3' miRNA: 3'- -CGGGaAGa--------GAGGGu----CUGGGGa-GGAGA- -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 16917 | 0.66 | 0.801871 |
Target: 5'- aGCUCgccgUCU-CCGGcCCCCUCCUCg -3' miRNA: 3'- -CGGGaag-AGAgGGUCuGGGGAGGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 39004 | 0.66 | 0.801871 |
Target: 5'- cGCCCcccCUCccccggCCCGGaACCCCgaaaagCCUCg -3' miRNA: 3'- -CGGGaa-GAGa-----GGGUC-UGGGGa-----GGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 8103 | 0.66 | 0.801871 |
Target: 5'- cGCCCcccCUCccccggCCCGGaACCCCgaaaagCCUCg -3' miRNA: 3'- -CGGGaa-GAGa-----GGGUC-UGGGGa-----GGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 54545 | 0.66 | 0.801871 |
Target: 5'- uGCCCggCgcgCCCGGcgcgGCCCCggCCUCg -3' miRNA: 3'- -CGGGaaGagaGGGUC----UGGGGa-GGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 86637 | 0.66 | 0.793951 |
Target: 5'- aCCCgUCUCcaccagagcccccggCCaCAGACCCCcCCUCg -3' miRNA: 3'- cGGGaAGAGa--------------GG-GUCUGGGGaGGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 49840 | 0.66 | 0.793065 |
Target: 5'- cGCCCcg--CUCCgAcgccGCCCCUCCUCc -3' miRNA: 3'- -CGGGaagaGAGGgUc---UGGGGAGGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 151325 | 0.66 | 0.784119 |
Target: 5'- --gCUUCUcCUCCCGGGCCUCcccccgCCUCc -3' miRNA: 3'- cggGAAGA-GAGGGUCUGGGGa-----GGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 31431 | 0.66 | 0.784119 |
Target: 5'- gGCCCgcgCg--CCCGGGCCCUcccgCCUCc -3' miRNA: 3'- -CGGGaa-GagaGGGUCUGGGGa---GGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 530 | 0.66 | 0.784119 |
Target: 5'- gGCCCgcgCg--CCCGGGCCCUcccgCCUCc -3' miRNA: 3'- -CGGGaa-GagaGGGUCUGGGGa---GGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 120424 | 0.66 | 0.784119 |
Target: 5'- --gCUUCUcCUCCCGGGCCUCcccccgCCUCc -3' miRNA: 3'- cggGAAGA-GAGGGUCUGGGGa-----GGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 80420 | 0.66 | 0.784119 |
Target: 5'- uCCCUUCUUUCCCu--CCCCgCCg-- -3' miRNA: 3'- cGGGAAGAGAGGGucuGGGGaGGaga -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 75987 | 0.66 | 0.775043 |
Target: 5'- cGCCCcUCUCcaCCgCGGACCCC-CCa-- -3' miRNA: 3'- -CGGGaAGAGa-GG-GUCUGGGGaGGaga -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 89149 | 0.66 | 0.775043 |
Target: 5'- cCCCgcCUCcgUCCUGGugCgCCUCCUCg -3' miRNA: 3'- cGGGaaGAG--AGGGUCugG-GGAGGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 120964 | 0.66 | 0.763062 |
Target: 5'- cGCCUcUCUCucugucuccUCCCGGgcgagcgggcacacGCCCCgUCCUCg -3' miRNA: 3'- -CGGGaAGAG---------AGGGUC--------------UGGGG-AGGAGa -5' |
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21376 | 3' | -59 | NC_004812.1 | + | 151865 | 0.66 | 0.763062 |
Target: 5'- cGCCUcUCUCucugucuccUCCCGGgcgagcgggcacacGCCCCgUCCUCg -3' miRNA: 3'- -CGGGaAGAG---------AGGGUC--------------UGGGG-AGGAGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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