Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21778 | 3' | -51.7 | NC_004813.1 | + | 56418 | 0.7 | 0.701633 |
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Target: 5'- gUGCGGGUugAGuguGUACCGUUacgcUgAGCa -3' miRNA: 3'- gGCGCCUAugUCu--CGUGGCAA----AgUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 31698 | 0.7 | 0.734065 |
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Target: 5'- gCGUGGccauccGUGCGGAGCGCUGgaUCAacugGCg -3' miRNA: 3'- gGCGCC------UAUGUCUCGUGGCaaAGU----CG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 46529 | 0.7 | 0.734065 |
|
Target: 5'- aCGCGGAUaucaGCGGAGCaauuACCGcgaauacCGGCa -3' miRNA: 3'- gGCGCCUA----UGUCUCG----UGGCaaa----GUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 22384 | 0.69 | 0.775783 |
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Target: 5'- gCCaGUGGcgGCAGGGCAgaaGUggUCAGCg -3' miRNA: 3'- -GG-CGCCuaUGUCUCGUgg-CAa-AGUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 29990 | 0.69 | 0.775783 |
|
Target: 5'- -gGCGGAguuugGCAGuGCGcucCCGUUUCucuGGCa -3' miRNA: 3'- ggCGCCUa----UGUCuCGU---GGCAAAG---UCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 29123 | 0.68 | 0.842503 |
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Target: 5'- gUGCGGAUuucugggagcaGCAGGGCAggCGcgUCAGCc -3' miRNA: 3'- gGCGCCUA-----------UGUCUCGUg-GCaaAGUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 45587 | 1.16 | 0.000978 |
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Target: 5'- aCCGCGGAUACAGAGCACCGUUUCAGCg -3' miRNA: 3'- -GGCGCCUAUGUCUCGUGGCAAAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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