Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21778 | 3' | -51.7 | NC_004813.1 | + | 32605 | 0.69 | 0.765553 |
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Target: 5'- gUCGUGGAUcacuucccggGCAG-GCACCGUcaccgccUCAGUg -3' miRNA: 3'- -GGCGCCUA----------UGUCuCGUGGCAa------AGUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 34382 | 0.68 | 0.833572 |
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Target: 5'- cCUGCGGAgccgGCAGGGCacucACCGcauccagaaCAGCc -3' miRNA: 3'- -GGCGCCUa---UGUCUCG----UGGCaaa------GUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 41855 | 0.68 | 0.842503 |
|
Target: 5'- aCCG-GGAaACAGcGCACCGUccgucucggUCGGUg -3' miRNA: 3'- -GGCgCCUaUGUCuCGUGGCAa--------AGUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 32952 | 0.67 | 0.851207 |
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Target: 5'- gCCG-GGAcACGGAaCACCGaacagacaaUUUCAGCg -3' miRNA: 3'- -GGCgCCUaUGUCUcGUGGC---------AAAGUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 2655 | 0.66 | 0.898225 |
|
Target: 5'- uCUGCGcGAgcUACAgGAGCGCCGcaa-GGCu -3' miRNA: 3'- -GGCGC-CU--AUGU-CUCGUGGCaaagUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 14298 | 0.66 | 0.905133 |
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Target: 5'- aCGCGGAU----AGCAUCGUg-CGGCa -3' miRNA: 3'- gGCGCCUAugucUCGUGGCAaaGUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 44354 | 0.72 | 0.61292 |
|
Target: 5'- aCCGCGGAUAUGGuAGUucACCGUcaUCuGCu -3' miRNA: 3'- -GGCGCCUAUGUC-UCG--UGGCAa-AGuCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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