Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
218 | 3' | -64.1 | AC_000008.1 | + | 16960 | 0.66 | 0.227617 |
Target: 5'- --aGGCUgaguacgucucgguGGCGCGggcaaacugcacCAGCCCGGGGCu- -3' miRNA: 3'- ggaCCGG--------------UCGCGC------------GUCGGGCCUCGcg -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 4615 | 0.66 | 0.223582 |
Target: 5'- --gGGCCcacgGGCG-GCGGCCUGG-GCGa -3' miRNA: 3'- ggaCCGG----UCGCgCGUCGGGCCuCGCg -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 21845 | 0.66 | 0.217923 |
Target: 5'- --cGGCCGGuCGCG-AGaCUgGGGGCGUa -3' miRNA: 3'- ggaCCGGUC-GCGCgUC-GGgCCUCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 810 | 0.66 | 0.217923 |
Target: 5'- ---cGCCGGCGCcCGGUucuCCGGAGcCGCc -3' miRNA: 3'- ggacCGGUCGCGcGUCG---GGCCUC-GCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 23790 | 0.66 | 0.212387 |
Target: 5'- -aUGGCCGcgggcuggguGUGCGCGGCaCC--AGCGCg -3' miRNA: 3'- ggACCGGU----------CGCGCGUCG-GGccUCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 9454 | 0.66 | 0.206972 |
Target: 5'- --gGGUCGGCGaacggGCAGUgCCGGcGGCGCc -3' miRNA: 3'- ggaCCGGUCGCg----CGUCG-GGCC-UCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 16334 | 0.66 | 0.206972 |
Target: 5'- --cGGCCGcCGCaGCAGCCgCGGccauuAGUGCu -3' miRNA: 3'- ggaCCGGUcGCG-CGUCGG-GCC-----UCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 24890 | 0.66 | 0.206972 |
Target: 5'- uCgUGcGCC-GUGCGCAGCCCcuGGAGagggaUGCa -3' miRNA: 3'- -GgAC-CGGuCGCGCGUCGGG--CCUC-----GCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 7207 | 0.66 | 0.206972 |
Target: 5'- gCCUGGUaGGCGCaGCAuCCCuuuucuacgGGuAGCGCg -3' miRNA: 3'- -GGACCGgUCGCG-CGUcGGG---------CC-UCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 30266 | 0.66 | 0.205372 |
Target: 5'- aCCUGGCUcguGGaaugcgagaccgucUGCGgGGCCUGcGGCGCg -3' miRNA: 3'- -GGACCGG---UC--------------GCGCgUCGGGCcUCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 23195 | 0.67 | 0.196501 |
Target: 5'- ---aGCCAcaaCGCGCAGCCCGuGGGCu- -3' miRNA: 3'- ggacCGGUc--GCGCGUCGGGC-CUCGcg -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 17115 | 0.67 | 0.196501 |
Target: 5'- cCCgaGGUCcGCGUGCGGCCaaucaagcaGGuGGCGCc -3' miRNA: 3'- -GGa-CCGGuCGCGCGUCGGg--------CC-UCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 22264 | 0.67 | 0.186495 |
Target: 5'- uCCgcaGCCacAGUGCGCAGauuaGGAGCGCc -3' miRNA: 3'- -GGac-CGG--UCGCGCGUCggg-CCUCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 27232 | 0.67 | 0.186495 |
Target: 5'- uCCU-GCCcGCGCGCGGCgCCGacGUGCc -3' miRNA: 3'- -GGAcCGGuCGCGCGUCG-GGCcuCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 23718 | 0.67 | 0.180234 |
Target: 5'- uCC-GGCCccagcugccuccagGGCGCGuCGGCuuGGggcccAGCGCa -3' miRNA: 3'- -GGaCCGG--------------UCGCGC-GUCGggCC-----UCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 17394 | 0.67 | 0.176941 |
Target: 5'- gCUGGcCCAGCacuccgGUAGCCauGGGCGCg -3' miRNA: 3'- gGACC-GGUCGcg----CGUCGGgcCUCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 15967 | 0.68 | 0.162991 |
Target: 5'- --cGGCgCGGgcacuacCGCGC-GCCCuGGGGCGCg -3' miRNA: 3'- ggaCCG-GUC-------GCGCGuCGGG-CCUCGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 16109 | 0.68 | 0.158707 |
Target: 5'- --cGGCCAuucagaccgugguGCGCGgAGCCCGGcGCu- -3' miRNA: 3'- ggaCCGGU-------------CGCGCgUCGGGCCuCGcg -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 24406 | 0.68 | 0.150442 |
Target: 5'- --cGGCCGccacgugugcGCGCGCGGgacuaauCCCGGuucGCGCg -3' miRNA: 3'- ggaCCGGU----------CGCGCGUC-------GGGCCu--CGCG- -5' |
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218 | 3' | -64.1 | AC_000008.1 | + | 19891 | 0.69 | 0.139152 |
Target: 5'- --cGGCCGcguuuGUGCGCGcCCCaGGGCGCg -3' miRNA: 3'- ggaCCGGU-----CGCGCGUcGGGcCUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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