Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22250 | 3' | -57.3 | NC_005045.1 | + | 28675 | 0.66 | 0.500186 |
Target: 5'- aGCaGGCGCACUCCAUCcaggcucaggcuaagGCCGGc--- -3' miRNA: 3'- cCGgCCGUGUGAGGUAG---------------CGGCUcuuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 28975 | 0.69 | 0.361326 |
Target: 5'- aGUC-GCGCACUCCG-CGCCGAGc-- -3' miRNA: 3'- cCGGcCGUGUGAGGUaGCGGCUCuuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 29516 | 0.7 | 0.281933 |
Target: 5'- uGCCGGgACAgUUCGUCGCCGGu--- -3' miRNA: 3'- cCGGCCgUGUgAGGUAGCGGCUcuuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 34283 | 0.67 | 0.435212 |
Target: 5'- uGCCGGCGuaguuCUCCAUCaGCCGGucGAGGu -3' miRNA: 3'- cCGGCCGUgu---GAGGUAG-CGGCU--CUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 35883 | 0.69 | 0.34427 |
Target: 5'- --aCGGCGUACUCgacgcgauccgCAUCGCCGAGGAAg -3' miRNA: 3'- ccgGCCGUGUGAG-----------GUAGCGGCUCUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 38437 | 0.69 | 0.352725 |
Target: 5'- cGCUGGaGgGCUUCAUCGCCGGuGAGAc -3' miRNA: 3'- cCGGCCgUgUGAGGUAGCGGCU-CUUU- -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 40064 | 0.67 | 0.445048 |
Target: 5'- uGCUGGCaggACACguggucggaaUCCAUCGCCGAu--- -3' miRNA: 3'- cCGGCCG---UGUG----------AGGUAGCGGCUcuuu -5' |
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22250 | 3' | -57.3 | NC_005045.1 | + | 40331 | 0.67 | 0.435212 |
Target: 5'- cGGCCcuuGCgACGCUCCcggcccaUGCCGAGAGAc -3' miRNA: 3'- -CCGGc--CG-UGUGAGGua-----GCGGCUCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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