Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22250 | 3' | -57.3 | NC_005045.1 | + | 28975 | 0.69 | 0.361326 |
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Target: 5'- aGUC-GCGCACUCCG-CGCCGAGc-- -3' miRNA: 3'- cCGGcCGUGUGAGGUaGCGGCUCuuu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 4638 | 0.69 | 0.34427 |
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Target: 5'- aGGCUGGCGCACcagCCuUCGCCuuGGu- -3' miRNA: 3'- -CCGGCCGUGUGa--GGuAGCGGcuCUuu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 5014 | 0.69 | 0.3278 |
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Target: 5'- uGGCCGGUGCGCU--GUCGCUGAa--- -3' miRNA: 3'- -CCGGCCGUGUGAggUAGCGGCUcuuu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 29516 | 0.7 | 0.281933 |
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Target: 5'- uGCCGGgACAgUUCGUCGCCGGu--- -3' miRNA: 3'- cCGGCCgUGUgAGGUAGCGGCUcuuu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 22292 | 0.73 | 0.17469 |
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Target: 5'- aGCuCGGCGUACUCCAUCGCCGuGc-- -3' miRNA: 3'- cCG-GCCGUGUGAGGUAGCGGCuCuuu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 10888 | 0.78 | 0.086103 |
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Target: 5'- uGGCCGa-GCGCUCCAUCGCgGAGAc- -3' miRNA: 3'- -CCGGCcgUGUGAGGUAGCGgCUCUuu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 11909 | 1.08 | 0.000507 |
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Target: 5'- aGGCCGGCACACUCCAUCGCCGAGAAAc -3' miRNA: 3'- -CCGGCCGUGUGAGGUAGCGGCUCUUU- -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 5836 | 0.66 | 0.527796 |
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Target: 5'- cGUCGGCGCGCUUCG-CGUCGGc--- -3' miRNA: 3'- cCGGCCGUGUGAGGUaGCGGCUcuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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