Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22250 | 3' | -57.3 | NC_005045.1 | + | 22715 | 0.67 | 0.435212 |
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Target: 5'- uGGgCGGCACauACUCgCucuUgGCCGAGAAc -3' miRNA: 3'- -CCgGCCGUG--UGAG-Gu--AgCGGCUCUUu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 18612 | 0.67 | 0.442084 |
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Target: 5'- gGGgCGGCACGagcguccaccuggagCCGUCGCCG-GAGg -3' miRNA: 3'- -CCgGCCGUGUga-------------GGUAGCGGCuCUUu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 18212 | 0.67 | 0.455008 |
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Target: 5'- gGGCCGGCAgccaaGCUCCGgcacUUGCUGcGGAu -3' miRNA: 3'- -CCGGCCGUg----UGAGGU----AGCGGCuCUUu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 4513 | 0.66 | 0.485585 |
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Target: 5'- cGGgUGGCGCAUggUCC-UCGCCGAcaaGggGa -3' miRNA: 3'- -CCgGCCGUGUG--AGGuAGCGGCU---CuuU- -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 4822 | 0.66 | 0.485585 |
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Target: 5'- cGCCGaGCGcCugUCCAgccucgUCGCCG-GAGAc -3' miRNA: 3'- cCGGC-CGU-GugAGGU------AGCGGCuCUUU- -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 28675 | 0.66 | 0.500186 |
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Target: 5'- aGCaGGCGCACUCCAUCcaggcucaggcuaagGCCGGc--- -3' miRNA: 3'- cCGgCCGUGUGAGGUAG---------------CGGCUcuuu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 13221 | 0.66 | 0.505448 |
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Target: 5'- gGGCCGGauggcaaCugGCgCCAguacCGCCGGGGAu -3' miRNA: 3'- -CCGGCC-------GugUGaGGUa---GCGGCUCUUu -5' |
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| 22250 | 3' | -57.3 | NC_005045.1 | + | 5297 | 0.73 | 0.179549 |
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Target: 5'- cGCCGaGCGCGCa-CGUCGCUGGGAGAu -3' miRNA: 3'- cCGGC-CGUGUGagGUAGCGGCUCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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