Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22312 | 5' | -55.9 | NC_005045.1 | + | 19783 | 0.67 | 0.53202 |
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Target: 5'- cGCGCCUcGCCUUccGCGCCagGCGcUCGGu -3' miRNA: 3'- aCGCGGA-UGGAGu-UGCGGg-UGU-AGCU- -5' |
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| 22312 | 5' | -55.9 | NC_005045.1 | + | 27590 | 0.66 | 0.564843 |
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Target: 5'- gGCGCCUACUUCAcugGCGacaaCCugAUgGu -3' miRNA: 3'- aCGCGGAUGGAGU---UGCg---GGugUAgCu -5' |
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| 22312 | 5' | -55.9 | NC_005045.1 | + | 17719 | 0.66 | 0.564843 |
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Target: 5'- aGC-CCUugACCUCGGCGgCCCACugcaucaugCGAg -3' miRNA: 3'- aCGcGGA--UGGAGUUGC-GGGUGua-------GCU- -5' |
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| 22312 | 5' | -55.9 | NC_005045.1 | + | 36010 | 0.66 | 0.575907 |
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Target: 5'- gGCGCagcGCCUCAuUGgCgGCGUCGAg -3' miRNA: 3'- aCGCGga-UGGAGUuGCgGgUGUAGCU- -5' |
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| 22312 | 5' | -55.9 | NC_005045.1 | + | 39103 | 0.66 | 0.575907 |
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Target: 5'- cUGCGaCUGCCgugUCAGCuucGCCCGC-UCGGu -3' miRNA: 3'- -ACGCgGAUGG---AGUUG---CGGGUGuAGCU- -5' |
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| 22312 | 5' | -55.9 | NC_005045.1 | + | 33681 | 0.66 | 0.575907 |
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Target: 5'- cUGcCGCCgACCaUCAAgGCCCugGcCGAc -3' miRNA: 3'- -AC-GCGGaUGG-AGUUgCGGGugUaGCU- -5' |
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| 22312 | 5' | -55.9 | NC_005045.1 | + | 8786 | 0.66 | 0.58702 |
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Target: 5'- cGCGCCUguaCUCGGCGgCCCugGguaUGAc -3' miRNA: 3'- aCGCGGAug-GAGUUGC-GGGugUa--GCU- -5' |
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| 22312 | 5' | -55.9 | NC_005045.1 | + | 41307 | 0.66 | 0.58702 |
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Target: 5'- aGCgGCCUAUCagGACGCCCugGa--- -3' miRNA: 3'- aCG-CGGAUGGagUUGCGGGugUagcu -5' |
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| 22312 | 5' | -55.9 | NC_005045.1 | + | 15473 | 0.66 | 0.608233 |
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Target: 5'- gGCGCCccGCCUCGcucguacACGUCCGCcaCGGc -3' miRNA: 3'- aCGCGGa-UGGAGU-------UGCGGGUGuaGCU- -5' |
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| 22312 | 5' | -55.9 | NC_005045.1 | + | 39643 | 0.66 | 0.620554 |
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Target: 5'- gUGCccaGCCUccaGCCaUCAGCGCCgGCAgguacagcUCGGg -3' miRNA: 3'- -ACG---CGGA---UGG-AGUUGCGGgUGU--------AGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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