Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22359 | 3' | -52.2 | NC_005045.1 | + | 9362 | 0.69 | 0.630267 |
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Target: 5'- aGCAU-AGCCGgGUGcUCGAAGGcaccaaagaaAUUCCa -3' miRNA: 3'- -CGUGuUCGGCgUAC-AGCUUCC----------UGAGG- -5' |
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| 22359 | 3' | -52.2 | NC_005045.1 | + | 24547 | 0.69 | 0.61886 |
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Target: 5'- aGCuc-GGCgacuuccuugCGCAUGUCGAAGGAUugggUCCa -3' miRNA: 3'- -CGuguUCG----------GCGUACAGCUUCCUG----AGG- -5' |
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| 22359 | 3' | -52.2 | NC_005045.1 | + | 13965 | 0.7 | 0.607466 |
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Target: 5'- cGCACGGGuuGUggGUCGAAcucGGACgUUCu -3' miRNA: 3'- -CGUGUUCggCGuaCAGCUU---CCUG-AGG- -5' |
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| 22359 | 3' | -52.2 | NC_005045.1 | + | 29441 | 0.7 | 0.562216 |
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Target: 5'- cCACGGGCgGCcugGUCGAuGGACcagUCCa -3' miRNA: 3'- cGUGUUCGgCGua-CAGCUuCCUG---AGG- -5' |
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| 22359 | 3' | -52.2 | NC_005045.1 | + | 34258 | 0.72 | 0.48458 |
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Target: 5'- aGCAUuGGCCcgcgcagGUAUGcCGggGGACUCg -3' miRNA: 3'- -CGUGuUCGG-------CGUACaGCuuCCUGAGg -5' |
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| 22359 | 3' | -52.2 | NC_005045.1 | + | 22159 | 0.72 | 0.454343 |
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Target: 5'- cGCGCAGGCCGCAaccUGUcCGGuucGGGCagCg -3' miRNA: 3'- -CGUGUUCGGCGU---ACA-GCUu--CCUGagG- -5' |
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| 22359 | 3' | -52.2 | NC_005045.1 | + | 32427 | 0.75 | 0.332867 |
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Target: 5'- cUugAAGCCGCGUG-CGgcGGcCUCCu -3' miRNA: 3'- cGugUUCGGCGUACaGCuuCCuGAGG- -5' |
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| 22359 | 3' | -52.2 | NC_005045.1 | + | 30778 | 1.15 | 0.000639 |
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Target: 5'- aGCACAAGCCGCAUGUCGAAGGACUCCu -3' miRNA: 3'- -CGUGUUCGGCGUACAGCUUCCUGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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