Results 21 - 36 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22404 | 3' | -59 | NC_005056.1 | + | 8955 | 0.67 | 0.315672 |
Target: 5'- aUCGCUGUCACCGUCAGGGC-GUCGgCg -3' miRNA: 3'- -GGUGGUAGUGGUGGUCUCGgCGGCgG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 7662 | 0.67 | 0.32276 |
Target: 5'- gCCACUgaguaugugcuggAaCGCUacggGCgGGAGCUGCCGCCu -3' miRNA: 3'- -GGUGG-------------UaGUGG----UGgUCUCGGCGGCGG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 1397 | 0.67 | 0.331583 |
Target: 5'- aCGCag-CGCCACCuauGAgGCCaGCCGCa -3' miRNA: 3'- gGUGguaGUGGUGGu--CU-CGG-CGGCGg -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 16688 | 0.67 | 0.331583 |
Target: 5'- aCACCcgCACCGCgCAGGuGCUgGCCGaCa -3' miRNA: 3'- gGUGGuaGUGGUG-GUCU-CGG-CGGCgG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 1311 | 0.67 | 0.339757 |
Target: 5'- -aGCCAcUCACCGCCGGuaucAGCaCaCCGCUg -3' miRNA: 3'- ggUGGU-AGUGGUGGUC----UCG-GcGGCGG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 18906 | 0.67 | 0.339757 |
Target: 5'- uUCGCCAgUGCCauauucagcgugACCGGcGuuGCCGCCu -3' miRNA: 3'- -GGUGGUaGUGG------------UGGUCuCggCGGCGG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 5977 | 0.67 | 0.348076 |
Target: 5'- uUCACCcUCACUgACCGGcguGCCGCC-CUg -3' miRNA: 3'- -GGUGGuAGUGG-UGGUCu--CGGCGGcGG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 20709 | 0.67 | 0.353986 |
Target: 5'- gCAUCGUCAgcuucCCGCCGGucaguucgggauaaAGCaCGCCgGCCa -3' miRNA: 3'- gGUGGUAGU-----GGUGGUC--------------UCG-GCGG-CGG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 21212 | 0.66 | 0.365147 |
Target: 5'- uUCACCGUUGaugagguugaaCACCGGGGCgCGCCGg- -3' miRNA: 3'- -GGUGGUAGUg----------GUGGUCUCG-GCGGCgg -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 5787 | 0.66 | 0.365147 |
Target: 5'- -aGCUGaaACCgaaGCCGGAGCCGCaGCCc -3' miRNA: 3'- ggUGGUagUGG---UGGUCUCGGCGgCGG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 14190 | 0.66 | 0.373015 |
Target: 5'- gCACCAUC-CUuaaacauccauuuACCAGAGUCaucaGCaCGCCg -3' miRNA: 3'- gGUGGUAGuGG-------------UGGUCUCGG----CG-GCGG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 8247 | 0.66 | 0.373896 |
Target: 5'- gCGCCggUugCACCAGcGCC-CgGCCu -3' miRNA: 3'- gGUGGuaGugGUGGUCuCGGcGgCGG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 10031 | 0.66 | 0.382787 |
Target: 5'- uUACCGuuugaugagaguUCACCGCCGGuaugcgcgauGuuGCCGCg -3' miRNA: 3'- gGUGGU------------AGUGGUGGUCu---------CggCGGCGg -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 21573 | 0.66 | 0.391817 |
Target: 5'- -gGCCGUCACCcCCu--GCC-CCGCUu -3' miRNA: 3'- ggUGGUAGUGGuGGucuCGGcGGCGG- -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 11867 | 0.66 | 0.391817 |
Target: 5'- -aACCGUUAUCACCAcuGCCGgUGCa -3' miRNA: 3'- ggUGGUAGUGGUGGUcuCGGCgGCGg -5' |
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22404 | 3' | -59 | NC_005056.1 | + | 5618 | 0.66 | 0.400062 |
Target: 5'- uCCACagacUCACCGgCAGcgugggcgcgcauGGCCGCaagCGCCa -3' miRNA: 3'- -GGUGgu--AGUGGUgGUC-------------UCGGCG---GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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