Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22404 | 5' | -52 | NC_005056.1 | + | 8243 | 0.67 | 0.660585 |
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Target: 5'- cUGuGCGCCGGUUGCAccAGCGccCGGCc -3' miRNA: 3'- uGC-CGUGGCCGACGUuuUUGUu-GCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 12758 | 0.67 | 0.67234 |
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Target: 5'- cUGGCGgCGGC-GCu-GGGCAAUGACc -3' miRNA: 3'- uGCCGUgGCCGaCGuuUUUGUUGCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 22262 | 0.66 | 0.695706 |
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Target: 5'- uGCGGCauGCUGGCauaAAGGACAAUGAa -3' miRNA: 3'- -UGCCG--UGGCCGacgUUUUUGUUGCUg -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 11925 | 0.66 | 0.718788 |
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Target: 5'- uACuGCACCGGCaGUGGuGAUAACGGu -3' miRNA: 3'- -UGcCGUGGCCGaCGUUuUUGUUGCUg -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 26250 | 0.66 | 0.72678 |
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Target: 5'- -aGGCACCGGUaaggcguuuaccggUGCuuauGCAugGAa -3' miRNA: 3'- ugCCGUGGCCG--------------ACGuuuuUGUugCUg -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 20725 | 0.66 | 0.730189 |
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Target: 5'- gAUGGCACCGGCaUGauuu----ACGGCa -3' miRNA: 3'- -UGCCGUGGCCG-ACguuuuuguUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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