Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22404 | 5' | -52 | NC_005056.1 | + | 20287 | 0.74 | 0.296246 |
Target: 5'- cACGGCACCGGCUggccGCucuuAUGugGACc -3' miRNA: 3'- -UGCCGUGGCCGA----CGuuuuUGUugCUG- -5' |
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22404 | 5' | -52 | NC_005056.1 | + | 20725 | 0.66 | 0.730189 |
Target: 5'- gAUGGCACCGGCaUGauuu----ACGGCa -3' miRNA: 3'- -UGCCGUGGCCG-ACguuuuuguUGCUG- -5' |
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22404 | 5' | -52 | NC_005056.1 | + | 22262 | 0.66 | 0.695706 |
Target: 5'- uGCGGCauGCUGGCauaAAGGACAAUGAa -3' miRNA: 3'- -UGCCG--UGGCCGacgUUUUUGUUGCUg -5' |
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22404 | 5' | -52 | NC_005056.1 | + | 22423 | 0.68 | 0.625188 |
Target: 5'- gACGGCAaCGGgUGCGGccuuacGGCGugGACu -3' miRNA: 3'- -UGCCGUgGCCgACGUUu-----UUGUugCUG- -5' |
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22404 | 5' | -52 | NC_005056.1 | + | 26250 | 0.66 | 0.72678 |
Target: 5'- -aGGCACCGGUaaggcguuuaccggUGCuuauGCAugGAa -3' miRNA: 3'- ugCCGUGGCCG--------------ACGuuuuUGUugCUg -5' |
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22404 | 5' | -52 | NC_005056.1 | + | 27632 | 0.68 | 0.601604 |
Target: 5'- cACuGCAgCGGCUGUuaccGCAugGGCg -3' miRNA: 3'- -UGcCGUgGCCGACGuuuuUGUugCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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